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Mouse Monoclonal ARK-2 antibody [13E8D3] (STJ97851)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:10000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
AURKB antibody, AIK2 antibody, AIM1 antibody, AIRK2 antibody, ARK2 antibody, STK1 antibody, STK12 antibody, STK5 antibody,|AIK2 antibody|IPL1 antibody|PPP1R48 antibody|Protein phosphatase 1 regulatory subunit 48 antibody|Serine/theronine kinase 12 antibody|Serine/threonine protein kinase 12 antibody|Serine/threonine-protein kinase 12 antibody|Serine/threonine-protein kinase aurora-B antibody|STK-1 antibody|STK1 antibody|STK12 antibody|STK5 antibody|Anti-Aurora B antibody (ab2254)
SCBT cat No: sc-65987|sc-393357|sc-14326|sc-14327|sc-25426|sc-271827|
ARK-2 Monoclonal Antibody
|Catalogue No.|| |
ARK-2 Monoclonal Antibody detects endogenous levels of ARK-2 protein.
Purified recombinant fragment of ARK-2 expressed in E Coli
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:10000
ARK-2 Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.
ARK-2 Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Aurora kinase B antibody, Aurora 1 antibody, Aurora- and IPL1-like midbody-associated protein 1 antibody, AIM-1 antibody, Aurora/IPL1-related kinase 2 antibody, ARK-2 antibody, Aurora-related kinase 2 antibody, STK-1 antibody, Serine/threonine-protein kinase 12 antibody, Serine/threonine-protein kinase 5 antibody, Serine/threonine-protein kinase aurora-B antibody
|Protein names|| |
Aurora kinase B , Aurora 1 , Aurora- and IPL1-like midbody-associated protein 1 , AIM-1 , Aurora/IPL1-related kinase 2 , ARK-2 , Aurora-related kinase 2 , STK-1 , Serine/threonine-protein kinase 12 , Serine/threonine-protein kinase 5 , Serine/threonine-protein kinase aurora-B
|Protein function|| |
Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis . AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, GSG2/Haspin, and histone H3. A positive feedback loop involving GSG2 and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at ‘Ser-10’ and ‘Ser-28’ during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between GSG2 and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGOL1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes. / ATP + a protein = ADP + a phosphoprotein. / Mg2+ / Activity is greatly increased when AURKB is within the CPC complex. In particular, AURKB-phosphorylated INCENP acts as an activator of AURKB. Positive feedback between GSG2 and AURKB contributes to CPC localization.
|Protein tissue specificity|| |
High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
|Involvement in disease|| |
Note: Disruptive regulation of expression is a possible mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
The phosphorylation of Thr-232 requires the binding to INCENP and occurs by means of an autophosphorylation mechanism. Thr-232 phosphorylation is indispensable for the AURKB kinase activity. / Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
|Protein cellular localization|| |
Nucleus / Chromosome / Chromosome > centromere / Cytoplasm > cytoskeleton > spindle / Midbody
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St John’s Laboratory Ltd.
|Product type|| |
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