Mouse Monoclonal ATF-2 antibody [4F12] (STJ97854)

$379.00

Reactivity: Human, Mouse
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal ATF-2 antibody [4F12] (STJ97854)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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ATF2 antibody, CREB2 antibody, CREBP1 antibody,|Activating transcription factor 2 antibody|Activating transcription factor 2 splice variant ATF2 var2 antibody|ATF 2 antibody|Atf-2 antibody|Atf2 antibody|ATF2 protein antibody|ATF2_HUMAN antibody|cAMP Response Element Binding Protein 2 antibody|cAMP response element binding protein CRE BP1 antibody|cAMP response element-binding protein CRE-BP1 antibody|cAMP responsive element binding protein 2, formerly antibody|cAMP-dependent transcription factor ATF-2 antibody|cAMP-responsive element-binding protein 2 antibody|CRE BP1 antibody|CRE-BP antibody|CREB 2 antibody|CREB-2 antibody|CREB2 antibody|CREBP1 antibody|Cyclic AMP dependent transcription factor ATF 2 antibody|Cyclic AMP-dependent transcription factor ATF-2 antibody|Cyclic AMP-responsive element-binding protein 2 antibody|D130078H02Rik antibody|D18875 antibody|HB 16 antibody|HB16 antibody|Histone acetyltransferase ATF2 antibody|MGC105211 antibody|MGC105222 antibody|MGC111558 antibody|MGC142504 antibody|mXBP antibody|MXBP protein antibody|Tg(Gzma-Klra1)7Wum antibody|TREB 7 antibody|TREB7 antibody|Phospho anti-ATF2 (T69 + T51) antibody (ab28848)
SCBT cat No: sc-187|sc-242|sc-6233|sc-81189|sc-188|sc-22798|sc-377168|

 

Name

ATF-2 Monoclonal Antibody

Catalogue No.

STJ97854

Reactivity

Human, Mouse

Specificity

ATF-2 Monoclonal Antibody detects endogenous levels of ATF-2 protein.

Immunogen

Purified recombinant fragment of human ATF-2 expressed in E Coli

Host

Mouse

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

ATF-2 Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.

Concentration

1 mg/ml

Purification

ATF-2 Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Cyclic AMP-dependent transcription factor ATF-2 antibody, cAMP-dependent transcription factor ATF-2 antibody, Activating transcription factor 2 antibody, Cyclic AMP-responsive element-binding protein 2 antibody, CREB-2 antibody, cAMP-responsive element-binding protein 2 antibody, HB16 antibody, Histone acetyltransferase ATF2 antibody, cAMP response element-binding protein CRE-BP1 antibody

Database links

Human UniProt/Swiss-Prot:P15336;Mouse UniPort/Swiss-Prot: P16951;Rat UniProt/Swiss-Port: Q00969;Human Entrez Gene: 1386;Mouse Entrez Gene: 11909;Rat Entrez Gene: Rn.9825

Protein names

Cyclic AMP-dependent transcription factor ATF-2 , cAMP-dependent transcription factor ATF-2 , Activating transcription factor 2 , Cyclic AMP-responsive element-binding protein 2 , CREB-2 , cAMP-responsive element-binding protein 2 , HB16 , Histone acetyltransferase ATF2 , cAMP response element-binding protein CRE-BP1

Protein function

Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5′-TGACGTCA-3′) or to AP-1 (activator protein 1) consensus sequences (5′-TGACTCA-3′). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type. / Acetyl-CoA + [histone] = CoA + acetyl-[histone].

Protein tissue specificity

Ubiquitously expressed, with more abundant expression in the brain.

Protein sequence and domain

The nuclear export signal 1 (N-NES) negatively regulates its nuclear localization and transcriptional activity. / Belongs to the bZIP family. ATF subfamily. / Contains 1 bZIP (basic-leucine zipper) domain. / Contains 1 C2H2-type zinc finger.

Protein post-translational modifications

Phosphorylation of Thr-69 by MAPK14 and MAPK11, and at Thr-71 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity. Phosphorylated by PLK3 following hyperosmotic stress. Also phosphorylated and activated by JNK and CaMK4. ATM-mediated phosphorylation at Ser-490 and Ser-498 stimulates its function in DNA damage response. Phosphorylation at Ser-62, Thr-73 and Ser-121 activates its transcriptional activity. Phosphorylation at Thr-69 or Thr-71 enhances its histone acetyltransferase (HAT) activity.

Protein cellular localization

Nucleus / Cytoplasm / Mitochondrion outer membrane

Research area

All research areas>Transcription Regulators>ATF
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize ATF-2 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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