Mouse Monoclonal Bax antibody [6F11] (STJ97725)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Bax antibody [6F11] (STJ97725)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC

Recommended dilution: WB 1:1000-2000; IHC 1:100-200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Bax Mouse Monoclonal Antibody(6F11).

Alternative names:

Check alternative names for the antibody

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BAX antibody, BCL2L4 antibody,|Apoptosis regulator BAX antibody|BAX antibody|Bax-protein antibody|BAX_HUMAN antibody|BAXA antibody|Baxdelta2G9 antibody|Baxdelta2G9omega antibody|Baxdelta2omega antibody|Bcl-2-like protein 4 antibody|BCL2 associated X protein antibody|BCL2 associated X protein omega antibody|BCL2 associated X protein transcript variant delta2 antibody|Bcl2-L-4 antibody|BCL2L4 antibody|membrane isoform alpha antibody|Anti-Bax antibody [E63] (ab32503)
SCBT cat No: sc-6236|sc-20067|sc-70407|sc-23959|sc-70405|sc-70408|sc-7480|sc-493|sc-526|sc-65532|sc-80658|sc-20288|

Name

Bax Mouse Monoclonal Antibody(6F11)

Catalogue No.

STJ97725

Reactivity

Human, Mouse, Rat

Specificity

Bax Mouse Monoclonal Antibody(6F11) detects endogenous levels of BAX

Immunogen

Synthetic Peptide of Bax at AA range of 30-110

Host

Mouse

Applications

WB, IHC

Recommended dilution

WB 1:1000-2000; IHC 1:100-200

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

Bax Antibody(6F11) was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Bax Mouse Antibody(6F11) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Apoptosis regulator BAX antibody, Bcl-2-like protein 4 antibody, Bcl2-L-4 antibody

Database links

Human UniProt/Swiss-Prot:Q07812;Mouse UniPort/Swiss-Prot: Q07813;Rat UniProt/Swiss-Port: G3V8T9;Human Entrez Gene: 581;Mouse Entrez Gene: 12028;Rat Entrez Gene: Rn.10668

Protein names

Apoptosis regulator BAX , Bcl-2-like protein 4 , Bcl2-L-4

Protein function

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Protein tissue specificity

Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt’s lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

Protein sequence and domain

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. / Belongs to the Bcl-2 family.

Protein cellular localization

Mitochondrion membrane; Single-pass membrane protein / Cytoplasm / Cytoplasm / Cytoplasm / Cytoplasm

Research area

All research areas>Tumor Suppressor/Apoptosis>Bax
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize Bax Antibody(6F11) according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human breast cancer tissue

1: Bax Mouse Monoclonal Antibody(6F11)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human breast cancer tissue

1: Bax Mouse Monoclonal Antibody(6F11)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human Tonsil tissue

1: Bax Mouse Monoclonal Antibody(6F11) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach tissue

1: Bax Mouse Monoclonal Antibody(6F11) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: Bax Mouse Monoclonal Antibody(6F11) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human appendix tissue

1: Bax Mouse Monoclonal Antibody(6F11) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat spleen tissue

1: Bax Mouse Monoclonal Antibody(6F11) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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