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Mouse Monoclonal Bcl-2 antibody (KLH) [8E12] (STJ97860)
Supplier: St John’s Laboratory Ltd.
Recommended applications: IHC, IF, FC, ELISA
Recommended dilution: IHC 1:200-1:1000; IF 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
BCL2 antibody,|Apoptosis regulator Bcl 2 antibody|Apoptosis regulator Bcl-2 antibody|Apoptosis regulator Bcl2 antibody|AW986256 antibody|B cell CLL/lymphoma 2 antibody|B cell leukemia/lymphoma 2 antibody|Bcl-2 antibody|Bcl2 antibody|BCL2_HUMAN antibody|C430015F12Rik antibody|D630044D05Rik antibody|D830018M01Rik antibody|Leukemia/lymphoma, B-cell, 2 antibody|Oncogene B-cell leukemia 2 antibody|PPP1R50 antibody|Protein phosphatase 1, regulatory subunit 50 antibody|Anti-Bcl-2 antibody [E17] (ab32124)
SCBT cat No: sc-509|sc-56015|sc-23960|sc-130307|sc-578|sc-70411|sc-130308|sc-65392|sc-783|sc-7382|sc-492|sc-56018|sc-81002|sc-169990|sc-169989|sc-32741|sc-185|sc-13038|sc-70414|
Bcl-2 Monoclonal Antibody
|Catalogue No.|| |
Bcl-2 Monoclonal Antibody detects endogenous levels of Bcl-2 protein.
Synthetic peptide corresponding to residues surrounding Bcl-2, conjugated to KLH
IHC, IF, FC, ELISA
|Recommended dilution|| |
IHC 1:200-1:1000; IF 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000
Bcl-2 Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.
Bcl-2 Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Apoptosis regulator Bcl-2 antibody
|Protein names|| |
Apoptosis regulator Bcl-2
|Protein function|| |
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release .
|Protein tissue specificity|| |
Expressed in a variety of tissues.
|Involvement in disease|| |
Note: A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. .
|Protein sequence and domain|| |
BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity. / The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3. / The loop between motifs BH4 and BH3 is required for the interaction with NLRP1. / Belongs to the Bcl-2 family.
|Protein post-translational modifications|| |
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). / Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. / Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
|Protein cellular localization|| |
Mitochondrion outer membrane / Single-pass membrane protein / Nucleus membrane / Single-pass membrane protein / Endoplasmic reticulum membrane / Single-pass membrane protein
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St John’s Laboratory Ltd.
|Product type|| |
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