Mouse Monoclonal Beclin-1 antibody [5C2] (STJ97761)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Beclin-1 antibody [5C2] (STJ97761)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC

Recommended dilution: WB 1:1000-2000; IHC 1:100-200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Beclin-1 Mouse Monoclonal Antibody(5C2).

Alternative names:

Check alternative names for the antibody

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BECN1 antibody, GT197 antibody,|APG6 antibody|Bcl-2-interacting protein beclin antibody|Beclin-1 antibody|Beclin1 antibody|BECN 1 antibody|Becn1 antibody|BECN1_HUMAN antibody|Coiled coil myosin like BCL2 interacting protein antibody|Coiled-coil myosin-like BCL2-interacting protein antibody|GT197 antibody|Protein GT197 antibody|VPS 30 antibody|VPS30 antibody|Anti-Beclin 1 antibody (ab62557)
SCBT cat No: sc-10086|sc-48341|sc-48381|sc-11427|sc-10087|

 

Name

Beclin-1 Mouse Monoclonal Antibody(5C2)

Catalogue No.

STJ97761

Reactivity

Human, Mouse, Rat

Specificity

Beclin-1 Mouse Monoclonal Antibody(5C2) detects endogenous levels of BECN1

Immunogen

Synthetic Peptide of Beclin-1 at AA range of 110-190

Host

Mouse

Applications

WB, IHC

Recommended dilution

WB 1:1000-2000; IHC 1:100-200

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

Beclin-1 Antibody(5C2) was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Beclin-1 Mouse Antibody(5C2) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Beclin-1 antibody, Coiled-coil myosin-like BCL2-interacting protein antibody, Protein GT197 antibody

Database links

Human UniProt/Swiss-Prot:Q14457;Mouse UniPort/Swiss-Prot: O88597;Rat UniProt/Swiss-Port: Q91XJ1;Human Entrez Gene: 8678;Mouse Entrez Gene: 56208;Rat Entrez Gene: Rn.2776

Protein names

Beclin-1 , Coiled-coil myosin-like BCL2-interacting protein , Protein GT197

Protein function

Plays a central role in autophagy . Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 . Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis . Protects against infection by a neurovirulent strain of Sindbis virus . May play a role in antiviral host defense. / Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.

Protein tissue specificity

Ubiquitous.

Protein sequence and domain

The coiled coil domain can form antiparallel homodimers and mediates dimerization with the coiled coil domains of ATG14 or UVRAG involved in the formation of PI3K complexes. / Belongs to the beclin family.

Protein post-translational modifications

Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy . In response to autophagic stimuli, phosphorylated at serine residues by AMPK in an ATG14-dependent manner, and this phosphorylation is critical for maximally efficient autophagy . / Polyubiquitinated by NEDD4, both with ‘Lys11’- and ‘Lys63’-linkages. ‘Lys’-11-linked poyubiquitination leads to degradation and is enhanced when the stabilizing interaction partner VPS34 is depleted. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes. / Proteolytically processed by caspases including CASP8 and CASP3; the C-terminal fragments lack autophagy-inducing capacity and are proposed to induce apoptosis. Thus the cleavage is proposed to be an determinant to switch from autophagy to apoptosis pathways affecting cellular homeostasis including viral infections and survival of tumor cells.

Protein cellular localization

Cytoplasm / Golgi apparatus > trans-Golgi network membrane / Peripheral membrane protein / Endosome membrane / Peripheral membrane protein / Endoplasmic reticulum membrane / Peripheral membrane protein / Mitochondrion membrane / Peripheral membrane protein / Endosome / Cytoplasmic vesicle > autophagosome / Mitochondrion / Nucleus / Cytoplasm / Mitochondrion

Research area

All research areas>Tumor Suppressor/Apoptosis>BECN
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize Beclin-1 Antibody(5C2) according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


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Antibody customer reviews & validation data - 27

Validation data ID: 27

Submission date: 2016-11-29

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Result figure:
Result figure

Figure description:

Rat thyrocytes were treated with TSH receptor antibody for 24 hrs., fixed, permeabilized and then stained with primary antibodies. After washing FAR red conjugated secondary antibody was used and then analyzed by LI-COR imaging.

Product name

Beclin-1, MAP LC3beta, LC3A, IL-6, IL-1beta

Product catalog No.

STJ97761, STJ97398, STJ97755, STJ96713, STJ96566

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Western Blot

Antibody specificity rating

Excellent

Antibody overall rating

Good

Your comments

Mouse Monoclonal Beclin-1 antibody [5C2] (STJ97761)
Rabbit Polyclonal MAP LC3beta antibody (STJ97398)
Mouse Monoclonal LC3A antibody [5G10] (STJ97755)
Rabbit Polyclonal IL-6 antibody (STJ96713)
Rabbit Polyclonal IL-1beta antibody (STJ96566)
In fact, I have used them in LI-COR IN CELL WESTERN method. They worked nicely. The method was according to the instructions provided with the LI-COR kit with their buffers and antibodies.

Detailed protocol

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