Mouse Monoclonal beta I tubulin antibody [3F7] (STJ96939)

$99.00$319.00

Reactivity: Human, Rat, Mouse
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal beta I tubulin antibody [3F7] (STJ96939)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB

Recommended dilution: WB 1:5000-10000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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TUBB1 antibody,|2810484G07Rik antibody|Beta tubulin 1, class VI antibody|Class VI beta tubulin antibody|dJ543J19.4 antibody|M(beta)1 antibody|TBB1_HUMAN antibody|TUBB1 antibody|Tubulin beta 1 class VI antibody|Tubulin beta-1 chain antibody|Tubulin, beta 1 antibody|tubulin, beta1 antibody|Anti-beta I Tubulin antibody [EPR16778] (ab179511)
SCBT cat No: To be updated

Name

beta I tubulin Monoclonal Antibody

Catalogue No.

STJ96939

Reactivity

Human, Rat, Mouse

Specificity

The antibody detects endgenousbeta I tubulin proteins.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

WB

Recommended dilution

WB 1:5000-10000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

beta I tubulin Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

beta I tubulin Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Tubulin beta-1 chain antibody

Database links

Human UniProt/Swiss-Prot:Q9H4B7;Mouse UniPort/Swiss-Prot: A2AQ07;Rat UniProt/Swiss-Port: M0R8B6;Human Entrez Gene: 81027;Mouse Entrez Gene: 545486;

Protein names

Tubulin beta-1 chain

Protein function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).

Protein tissue specificity

Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins.

Involvement in disease

Macrothrombocytopenia, autosomal dominant, TUBB1-related (MAD-TUBB1) [MIM:613112]: A congenital blood disorder characterized by increased platelet size and decreased number of circulating platelets. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the tubulin family.

Protein post-translational modifications

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group . Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold . / Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).

Protein cellular localization

Cytoplasm > cytoskeleton

Research area

All research areas>Structural Proteins>Tubulin
(View all antibody categories related to Structural Proteins)

Note

AntibodyPlus can customize beta I tubulin Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human breast cancer tissue

1: beta I tubulin Monoclonal Antibody(3F7)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human breast cancer tissue

1: beta I tubulin Monoclonal Antibody(3F7)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human colon cancer tissue

1: beta I tubulin Monoclonal Antibody(3F7)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human colon cancer tissue

1: beta I tubulin Monoclonal Antibody(3F7)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung cancer tissue

1: beta I tubulin Monoclonal Antibody(3F7)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung cancer tissue

1: beta I tubulin Monoclonal Antibody(3F7)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human uterus cancer tissue

1: beta I tubulin Monoclonal Antibody(3F7) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human colon tissue

1: beta I tubulin Monoclonal Antibody(3F7) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human appendix tissue

1: beta I tubulin Monoclonal Antibody(3F7) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat lung tissue

1: beta I tubulin Monoclonal Antibody(3F7) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: beta I tubulin Monoclonal Antibody(3F7) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse testis tissue

1: beta I tubulin Monoclonal Antibody(3F7) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse lung tissue

1: beta I tubulin Monoclonal Antibody(3F7) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue

1: beta I tubulin Monoclonal Antibody(3F7) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse brain tissue

1: beta I tubulin Monoclonal Antibody(3F7) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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