Mouse Monoclonal beta-Tubulin antibody [5G3] (STJ96932)

$99.00$319.00

Reactivity: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Sheep, Insect, Yeast
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal beta-Tubulin antibody [5G3] (STJ96932)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC

Recommended dilution: WB 1:5000; IHC 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for beta-Tubulin Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

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TUBB3 antibody, TUBB4 antibody,|beta 3 tubulin antibody|beta-4 antibody|CDCBM antibody|CDCBM1 antibody|CFEOM3 antibody|CFEOM3A antibody|FEOM3 antibody|M(beta)3 antibody|M(beta)6 antibody|MC1R antibody|Neuron specific beta III Tubulin antibody|Neuron-specific class III beta-tubulin antibody|QccE-11995 antibody|QccE-15186 antibody|TBB3_HUMAN antibody|Tubb 3 antibody|TUBB3 antibody|TUBB4 antibody|Tubulin beta 3 antibody|Tubulin beta 3 chain antibody|Tubulin beta 4 antibody|Tubulin beta III antibody|Tubulin beta-3 chain antibody|Tubulin beta-4 chain antibody|Tubulin beta-III antibody|Anti-beta III Tubulin antibody [2G10] (ab78078)
SCBT cat No: sc-80005|sc-69966|

Name

beta-Tubulin Monoclonal Antibody

Catalogue No.

STJ96932

Reactivity

Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Sheep, Insect, Yeast

Specificity

The antibody detects endogenous beta-tubulin protein.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

WB, IHC

Recommended dilution

WB 1:5000; IHC 1:200

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

beta-Tubulin Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Concentration

1 mg/ml

Purification

beta-Tubulin Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Tubulin beta-3 chain antibody, Tubulin beta-4 chain antibody, Tubulin beta-III antibody

Database links

Human UniProt/Swiss-Prot:Q13509;Mouse UniPort/Swiss-Prot: Q9ERD7;Rat UniProt/Swiss-Port: Q4QRB4;Human Entrez Gene: 10381;Mouse Entrez Gene: 22152;Rat Entrez Gene: Rn.43958

Protein names

Tubulin beta-3 chain , Tubulin beta-4 chain , Tubulin beta-III

Protein function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

Protein tissue specificity

Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.

Involvement in disease

Fibrosis of extraocular muscles, congenital, 3A (CFEOM3A) [MIM:600638]: A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases, the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Cortical dysplasia, complex, with other brain malformations 1 (CDCBM1) [MIM:614039]: A disorder of aberrant neuronal migration and disturbed axonal guidance. Affected individuals have mild to severe mental retardation, strabismus, axial hypotonia, and spasticity. Brain imaging shows variable malformations of cortical development, including polymicrogyria, gyral disorganization, and fusion of the basal ganglia, as well as thin corpus callosum, hypoplastic brainstem, and dysplastic cerebellar vermis. Extraocular muscles are not involved. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

The highly acidic C-terminal region may bind cations such as calcium. / Belongs to the tubulin family.

Protein post-translational modifications

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group . Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold . / Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). / Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

Protein cellular localization

Cytoplasm > cytoskeleton

Research area

All research areas>Structural Proteins>Tubulin
(View all antibody categories related to Structural Proteins)

Note

AntibodyPlus can customize beta-Tubulin Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human appendix tissue

1: beta-Tubulin Monoclonal Antibody(5G3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human appendix tissue

1: beta-Tubulin Monoclonal Antibody(5G3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human appendix tissue

1: beta-Tubulin Monoclonal Antibody(5G3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung cancer tissue

1: beta-Tubulin Monoclonal Antibody(5G3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung cancer tissue

1: beta-Tubulin Monoclonal Antibody(5G3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung cancer tissue

1: beta-Tubulin Monoclonal Antibody(5G3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse lung tissue

1: beta-Tubulin Monoclonal Antibody(5G3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse lung tissue

1: beta-Tubulin Monoclonal Antibody(5G3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse lung tissue

1: beta-Tubulin Monoclonal Antibody(5G3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human colon tissue

1: beta-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue

1: beta-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human appendix tissue

1: beta-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat heart tissue

1: beta-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat liver tissue

1: beta-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat lung tissue

1: beta-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat spleen tissue

1: beta-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse testis tissue

1: beta-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse lung tissue

1: beta-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue

1: beta-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse spleen tissue

1: beta-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse colon cancer tissue

1: beta-Tubulin Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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