Mouse Monoclonal beta-tubulin antibody (HRP) [5G3] (STJ97477)

$99.00$319.00

Reactivity: Human, Rat, Mouse, Monkey, Dog, Cow, Hamster, Rabbit, Sheep, Insect, Yeast
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Antibody Validation Project, Antibody Trial Sample, Antibody Free Sample for your own antibody validation and submitting antibody reviews, AntibodyPlus

Mouse Monoclonal beta-tubulin antibody (HRP) [5G3] (STJ97477)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC

Recommended dilution: WB 1:1000-1:3000; IHC 1:50-1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for beta-tubulin (HRP Conjugated) Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

Expand

TUBB antibody, TUBB5 antibody, OK/SW-cl.56 antibody,|Beta 4 tubulin antibody|Beta 5 tubulin antibody|BetaTubulin antibody|TBB5_HUMAN antibody|TUBB antibody|TUBB2 antibody|TUBB2A antibody|TUBB5 antibody|tubulin beta 2A antibody|Tubulin beta chain antibody|Tubulin beta-5 chain antibody|Anti-beta Tubulin antibody – Loading Control (ab6046)
SCBT cat No: sc-101527|sc-53140|sc-80011|sc-5274|sc-86255|sc-166729|sc-55529|sc-9104|sc-58882|sc-31782|sc-9935|sc-58884|

Name

beta-tubulin (HRP Conjugated) Monoclonal Antibody

Catalogue No.

STJ97477

Reactivity

Human, Rat, Mouse, Monkey, Dog, Cow, Hamster, Rabbit, Sheep, Insect, Yeast

Specificity

Thebeta-tubulin antibody can detects endogenousbeta-tubulin protein.

Immunogen

Recombinant Protein

Host

Mouse

Applications

WB, IHC

Recommended dilution

WB 1:1000-1:3000; IHC 1:50-1:200

Clonality

Monoclonal

Conjugation

HRP

Formulation

beta-tubulin (HRP Conjugated) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

beta-tubulin (HRP Conjugated) Antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Tubulin beta chain antibody, Tubulin beta-5 chain antibody

Database links

Human UniProt/Swiss-Prot:P07437;Mouse UniPort/Swiss-Prot: P99024;Rat UniProt/Swiss-Port: P69897;Human Entrez Gene: 203068;Mouse Entrez Gene: 22154;Rat Entrez Gene: Rn.2458

Protein names

Tubulin beta chain , Tubulin beta-5 chain

Protein function

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Protein tissue specificity

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Involvement in disease

Cortical dysplasia, complex, with other brain malformations 6 (CDCBM6) [MIM:615771]: A disorder of aberrant neuronal migration and disturbed axonal guidance. Affected individuals have microcephaly, ataxia, and severe delayed psychomotor development. Brain imaging shows variable malformations of cortical development, including white matter streaks, dysmorphic basal ganglia, corpus callosum abnormalities, brainstem and cerebellar hypoplasia, cortical dysplasia, polymicrogyria. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Skin creases, congenital symmetric circumferential, 1 (CSCSC1) [MIM:156610]: An autosomal dominant disease characterized by multiple, symmetric, circumferential rings of folded skin, affecting primarily the limbs. Affected individuals also exhibit intellectual disability, cleft palate, and dysmorphic features. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

The highly acidic C-terminal region may bind cations such as calcium. / Belongs to the tubulin family.

Protein post-translational modifications

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group . Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold . / Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).

Protein cellular localization

Cytoplasm > cytoskeleton

Research area

All research areas>Structural Proteins>Tubulin
(View all antibody categories related to Structural Proteins)

Note

AntibodyPlus can customize beta-tubulin (HRP Conjugated) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews
 

Immunofluorescence analysis of Human lung cancer tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung cancer tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse testis tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse testis tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat kidney tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat kidney tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human Tonsil tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human colon tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat heart tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat liver tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat lung tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat spleen tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse heart tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse testis tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse liver tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse lung tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse spleen tissue

1: beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


Welcome to AntibodyPlus!