Mouse Monoclonal Blk antibody [1E6] (STJ97869)

$379.00

Reactivity: Human
Applications: WB, IF, FC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Blk antibody [1E6] (STJ97869)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IF, FC, ELISA

Recommended dilution: WB 1:500-1:2000; IF 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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BLK antibody,|B lymphocyte kinase antibody|B lymphoid tyrosine kinase antibody|Blk antibody|BLK nonreceptor tyrosine kinase antibody|BLK_HUMAN antibody|EC 2.7.10.2 antibody|MGC 10442 antibody|MGC10442 antibody|MODY 11 antibody|MODY11 antibody|P55 BLK antibody|p55-BLK antibody|Tyrosine kinase B lymphocyte specific antibody|Tyrosine protein kinase antibody|Tyrosine-protein kinase BLK antibody|Phospho anti-BLK (Y501) antibody (ab111889)
SCBT cat No: sc-65939|sc-65980|sc-938|sc-393996|sc-376597|sc-393960|sc-329|

 

Name

Blk Monoclonal Antibody

Catalogue No.

STJ97869

Reactivity

Human

Specificity

Blk Monoclonal Antibody detects endogenous levels of Blk protein.

Immunogen

Purified recombinant fragment of human Blk expressed in E Coli

Host

Mouse

Applications

WB, IF, FC, ELISA

Recommended dilution

WB 1:500-1:2000; IF 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

Blk Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.

Concentration

1 mg/ml

Purification

Blk Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Tyrosine-protein kinase Blk antibody, B lymphocyte kinase antibody, p55-Blk antibody

Database links

Human UniProt/Swiss-Prot:P51451;Mouse UniPort/Swiss-Prot: P16277;Rat UniProt/Swiss-Port: Q4KM97;Human Entrez Gene: 640;Mouse Entrez Gene: 12143;Rat Entrez Gene: Rn.20030

Protein names

Tyrosine-protein kinase Blk , B lymphocyte kinase , p55-Blk

Protein function

Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Specifically binds and phosphorylates CD79A at ‘Tyr-188’and ‘Tyr-199’, as well as CD79B at ‘Tyr-196’ and ‘Tyr-207’. Phosphorylates also the immunoglobulin G receptors FCGR2A, FCGR2B and FCGR2C. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation. Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation. In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / Antibody-mediated surface engagement of the B-cell antigen receptor (BCR) which results in the phosphorylation of BLK on tyrosine residues, stimulates the enzymatic activity.

Protein tissue specificity

Expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles.

Involvement in disease

Maturity-onset diabetes of the young 11 (MODY11) [MIM:613375]: A form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. / Contains 1 protein kinase domain. / Contains 1 SH2 domain. / Contains 1 SH3 domain.

Protein post-translational modifications

Phosphorylated on tyrosine residues after antibody-mediated surface engagement of the B-cell antigen receptor (BCR). / Ubiquitination of activated BLK by the UBE3A ubiquitin protein ligase leads to its degradation by the ubiquitin-proteasome pathway.

Protein cellular localization

Cell membrane

Research area

All research areas>Kinases and Phosphatases>Blk
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize Blk Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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