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Mouse Monoclonal Brk antibody [2H12B8] (STJ97883)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:10000
Recommended protocols: check protocols
Click or hover above images to see image description for Brk Monoclonal Antibody.
Check alternative names for the antibodyExpand
PTK6 antibody, BRK antibody,|Breast tumor kinase antibody|Breast tumour kinase antibody|Protein tyrosine kinase 6 antibody|Protein-tyrosine kinase 6 antibody|Ptk6 antibody|PTK6_HUMAN antibody|Tyrosine protein kinase BRK antibody|Tyrosine-protein kinase BRK antibody|Anti-Brk antibody (ab71515)
SCBT cat No: sc-916|
Brk Monoclonal Antibody
|Catalogue No.|| |
Brk Monoclonal Antibody detects endogenous levels of Brk protein.
Purified recombinant fragment of human Brk expressed in E Coli
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:10000
Brk Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.
Brk Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Protein-tyrosine kinase 6 antibody, Breast tumor kinase antibody, Tyrosine-protein kinase BRK antibody
|Protein names|| |
Protein-tyrosine kinase 6 , Breast tumor kinase , Tyrosine-protein kinase BRK
|Protein function|| |
Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways. / Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / Activated by EGF, NRG1 and IGF1. Inhibited by SOCS3 to phosphorylate STAT3. Stabilized in the inactive form by an association between the SH3 domain and the SH2-TK linker region. Interaction between Trp-184 within SH2-TK linker region and the catalytic domain appears essential for positive regulation of kinase activity. / 83 µM for ATP / 37 nmol/min/mg enzyme
|Protein tissue specificity|| |
Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Not expressed in breast or ovarian tissue but expressed in high percentage of breast and ovarian cancers. Also overexpressed in some metastatic melanomas, lymphomas, colon cancers, squamous cell carcinomas and prostate cancers. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 2 is present in prostate epithelial cell lines derived from normal prostate and prostate adenocarcinomas, as well as in a variety of cell lines.
|Protein sequence and domain|| |
The SH3 domain plays a major role in substrate interactions. The SH2 domain of PTK6 plays a role in protein-protein interactions, but is likely more important for the regulation of catalytic activity. / Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily. / Contains 1 protein kinase domain. / Contains 1 SH2 domain. / Contains 1 SH3 domain.
|Protein post-translational modifications|| |
Autophosphorylated. Autophosphorylation of Tyr-342 leads to an increase of kinase activity. Tyr-447 binds to the SH2 domain when phosphorylated and negatively regulates kinase activity.
|Protein cellular localization|| |
Cytoplasm / Nucleus / Cell projection > ruffle / Membrane
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St John’s Laboratory Ltd.
|Product type|| |
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