Mouse Monoclonal c-Fos antibody [6A3] (STJ97486)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal c-Fos antibody [6A3] (STJ97486)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB

Recommended dilution: WB 1:1000-1:3000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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FOS antibody, G0S7 antibody,||
SCBT cat No: To be updated

 

Name

c-Fos Monoclonal Antibody

Catalogue No.

STJ97486

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Protein

Host

Mouse

Applications

WB

Recommended dilution

WB 1:1000-1:3000

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

c-Fos Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

c-Fos Antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Proto-oncogene c-Fos antibody, Cellular oncogene fos antibody, G0/G1 switch regulatory protein 7 antibody

Database links

Human UniProt/Swiss-Prot:P01100;Mouse UniPort/Swiss-Prot: P01101;Rat UniProt/Swiss-Port: P12841;Human Entrez Gene: 2353;Mouse Entrez Gene: 14281;Rat Entrez Gene: Rn.103750

Protein names

Proto-oncogene c-Fos , Cellular oncogene fos , G0/G1 switch regulatory protein 7

Protein function

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

Protein tissue specificity

Expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, they undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the protein becomes nuclear.

Protein sequence and domain

Belongs to the bZIP family. Fos subfamily. / Contains 1 bZIP (basic-leucine zipper) domain.

Protein post-translational modifications

Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation (By similarity). / Constitutively sumoylated with SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232. / In quiescent cells, the small amount of FOS present is phosphorylated at Tyr-10 and Tyr-30 by SRC. This Tyr-phosphorylated form is cytosolic. In growing cells, dephosphorylated by PTPN2. Dephosphorylation leads to the association with endoplasmic reticulum membranes and activation of phospholipid synthesis.

Protein cellular localization

Nucleus / Endoplasmic reticulum / Cytoplasm > cytosol

Research area

All research areas>Transcription Regulators>c-Fos
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize c-Fos Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Antibody customer reviews & validation data - 123

Validation data ID: 123

Submission date: 2017-02-19

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Result figure:
Result figure

Figure description:

1-4 brain tissue lyastes

Product name

Mouse Monoclonal c-Fos antibody [6A3]

Product catalog No.

STJ97486

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Western Blot

Antibody specificity rating

Average

Antibody overall rating

Average

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