Mouse Monoclonal CA IX antibody [12F10] (STJ96978)

$99.00$319.00

Reactivity: Human
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal CA IX antibody [12F10] (STJ96978)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IP

Recommended dilution: WB 1:3000; IP 1:200

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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CA9 antibody, G250 antibody, MN antibody,|CA-IX antibody|CA9 antibody|CAH9_HUMAN antibody|CAIX antibody|Carbonate dehydratase IX antibody|Carbonic anhydrase 9 antibody|Carbonic anhydrase IX antibody|Carbonic dehydratase antibody|G250 antibody|Membrane antigen MN antibody|MN antibody|P54/58N antibody|pMW1 antibody|RCC associated protein G250 antibody|RCC-associated antigen G250 antibody|Renal cell carcinoma-associated antigen G250 antibody|Anti-Carbonic Anhydrase IX antibody (ab15086)
SCBT cat No: sc-365900|sc-25599|sc-25600|

Name

CA IX Monoclonal Antibody

Catalogue No.

STJ96978

Reactivity

Human

Specificity

The antibody detects endogenous CA IX proteins.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

WB, IP

Recommended dilution

WB 1:3000; IP 1:200

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

CA IX Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

CA IX Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Carbonic anhydrase 9 antibody, Carbonate dehydratase IX antibody, Carbonic anhydrase IX antibody, CA-IX antibody, CAIX antibody, Membrane antigen MN antibody, P54/58N antibody, Renal cell carcinoma-associated antigen G250 antibody, RCC-associated antigen G250 antibody, pMW1 antibody

Database links

Human UniProt/Swiss-Prot:Q16790;Mouse UniPort/Swiss-Prot: Q8VHB5;Rat UniProt/Swiss-Port: F7FL00;Human Entrez Gene: 768;Mouse Entrez Gene: 230099;Rat Entrez Gene: Rn.162391

Protein names

Carbonic anhydrase 9 , Carbonate dehydratase IX , Carbonic anhydrase IX , CA-IX , CAIX , Membrane antigen MN , P54/58N , Renal cell carcinoma-associated antigen G250 , RCC-associated antigen G250 , pMW1

Protein function

Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. / H2CO3 = CO2 + H2O. / Zn2+ / Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). / Optimum pH is 6.5.

Protein tissue specificity

Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

Protein sequence and domain

Belongs to the alpha-carbonic anhydrase family.

Protein post-translational modifications

Asn-346 bears high-mannose type glycan structures.

Protein cellular localization

Nucleus / Nucleus > nucleolus / Cell membrane / Single-pass type I membrane protein / Cell projection > microvillus membrane / Single-pass type I membrane protein

Research area

All research areas>Synthesis and Degradation>Carbonic Anhydrases
(View all antibody categories related to Synthesis and Degradation)

Note

AntibodyPlus can customize CA IX Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human liver cancer tissue

1: CA IX Monoclonal Antibody(12F10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: CA IX Monoclonal Antibody(12F10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human kidney tissue

1: CA IX Monoclonal Antibody(12F10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human kidney tissue

1: CA IX Monoclonal Antibody(12F10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue

1: CA IX Monoclonal Antibody(12F10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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