Mouse Monoclonal CaMKIV antibody (STJ98474)

$379.00

Reactivity: Human, Mouse, Rat, Chicken, Dog, Pig
Applications: WB
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal CaMKIV antibody (STJ98474)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB

Recommended dilution: WB 1:1000-1:2000

Recommended protocols: check protocols

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Alternative names:

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CAMK4 antibody, CAMK antibody, CAMK-GR antibody, CAMKIV antibody,|Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody|Brain Ca(2+) calmodulin dependent protein kinase type IV antibody|Brain Ca++-calmodulin dependent protein kinase type IV antibody|Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody|Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody|Calcium/calmodulin dependent protein kinase IV antibody|Calcium/calmodulin dependent protein kinase type IV antibody|Calcium/calmodulin-dependent protein kinase type IV antibody|CAM kinase 4 antibody|CAM kinase GR antibody|CAM kinase IV antibody|CAM kinase-GR antibody|CaMK 4 antibody|CAMK GR antibody|CaMK IV antibody|Camk4 antibody|CaMKGR antibody|IV antibody|KCC4_HUMAN antibody|MGC36771 antibody|Anti-CAMKIV antibody (ab3557)
SCBT cat No: sc-136249|sc-166156|sc-166157|sc-1545|sc-17762|sc-9036|sc-55501|

 

Name

CaMKIV Monoclonal Antibody

Catalogue No.

STJ98474

Reactivity

Human, Mouse, Rat, Chicken, Dog, Pig

Specificity

CaMKIV Monoclonal Antibody detects endogenous levels of CaMKIV protein.

Immunogen

Purified recombinant human CaMKIV protein fragments expressed in Ecoli

Host

Mouse

Applications

WB

Recommended dilution

WB 1:1000-1:2000

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

CaMKIV Antibody was tube-contained. Purified in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.

Concentration

1 mg/ml

Purification

CaMKIV Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Calcium/calmodulin-dependent protein kinase type IV antibody, CaMK IV antibody, CaM kinase-GR antibody

Database links

Human UniProt/Swiss-Prot:Q16566;Rat UniProt/Swiss-Port: P13234;Human Entrez Gene: 814;Mouse Entrez Gene: 12326;Rat Entrez Gene: Rn.11046

Protein names

Calcium/calmodulin-dependent protein kinase type IV , CaMK IV , CaM kinase-GR

Protein function

Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4+/CD8+ double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on ‘Ser-133’ in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18. / ATP + a protein = ADP + a phosphoprotein. / Activated by Ca2+/calmodulin. Binding of calmodulin results in conformational change that relieves intrasteric autoinhibition and allows phosphorylation of Thr-200 within the activation loop by CaMKK1 or CaMKK2. Phosphorylation of Thr-200 results in a 10-20-fold increase in total activity to generate Ca2+/calmodulin-independent activity. Autophosphorylation of the N-terminus Ser-12 and Ser-13 is required for full activation. Inactivated by protein phosphatase 2A (PPP2CA/PPP2CB) which dephosphorylates Thr-200, thereby terminating autonomous activity and helping to maintain the enzyme in its autoinhibited state.

Protein tissue specificity

Expressed in brain, thymus, CD4 T-cells, testis and epithelial ovarian cancer tissue.

Protein sequence and domain

The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate. / Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. / Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylated by CaMKK1 and CaMKK2 on Thr-200. Dephosphorylated by protein phosphatase 2A. Autophosphorylated on Ser-12 and Ser-13. / Glycosylation at Ser-189 modulates the phosphorylation of CaMK4 at Thr-200 and negatively regulates its activity toward CREB1 in basal conditions and during early inomycin stimulation.

Protein cellular localization

Cytoplasm / Nucleus

Research area

All research areas>Kinases and Phosphatases>CaMK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize CaMKIV Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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