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Mouse Monoclonal Caspase-8 antibody [1H11] (STJ97895)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, FC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
CASP8 antibody, MCH5 antibody,|ALPS2B antibody|Caspase-8 subunit p10 antibody|CED 3 antibody|FADD Like ICE antibody|FADD-homologous ICE/CED-3-like protease antibody|FADD-like ICE antibody|FLICE antibody|FLJ17672 antibody|ICE-like apoptotic protease 5 antibody|MACH alpha 1/2/3 protein antibody|MACH antibody|MACH beta 1/2/3/4 protein antibody|MCH5 antibody|MGC78473 antibody|MORT1 associated ced 3 homolog antibody|MORT1-associated CED-3 homolog antibody|OTTHUMP00000163717 antibody|OTTHUMP00000163720 antibody|OTTHUMP00000163724 antibody|OTTHUMP00000163725 antibody|OTTHUMP00000165062 antibody|OTTHUMP00000165063 antibody|OTTHUMP00000165064 antibody|OTTHUMP00000206552 antibody|OTTHUMP00000206582 antibody|Anti-Caspase-8 antibody (ab25901)
SCBT cat No: sc-81656|sc-81657|sc-70501|sc-73526|sc-70502|sc-81661|sc-81662|sc-56070|sc-6133|sc-56072|sc-56071|sc-6136|sc-393776|sc-5263|sc-166320|sc-166596|sc-7890|sc-6134|
Caspase-8 Monoclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat, Monkey
Caspase-8 Monoclonal Antibody detects endogenous levels of Caspase-8 protein.
Purified recombinant fragment of human Caspase-8 expressed in E Coli
WB, IHC, FC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000
Caspase-8 Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.
Caspase-8 Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Caspase-8 antibody, CASP-8 antibody, Apoptotic cysteine protease antibody, Apoptotic protease Mch-5 antibody, CAP4 antibody, FADD-homologous ICE/ced-3-like protease antibody, FADD-like ICE antibody, FLICE antibody, ICE-like apoptotic protease 5 antibody, MORT1-associated ced-3 homolog antibody, MACH antibody
|Protein names|| |
Caspase-8 , CASP-8 , Apoptotic cysteine protease , Apoptotic protease Mch-5 , CAP4 , FADD-homologous ICE/ced-3-like protease , FADD-like ICE , FLICE , ICE-like apoptotic protease 5 , MORT1-associated ced-3 homolog , MACH
|Protein function|| |
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-, -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. / Strict requirement for Asp at position P1 and has a preferred cleavage sequence of (Leu/Asp/Val)-Glu-Thr-Asp-, -(Gly/Ser/Ala). / Inhibited by the effector protein NleF that is produced by pathogenic E.coli; this inhibits apoptosis.
|Protein tissue specificity|| |
Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
|Involvement in disease|| |
Caspase-8 deficiency (CASP8D) [MIM:607271]: Disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization. . Note: The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex. / Belongs to the peptidase C14A family. / Contains 2 DED (death effector) domains.
|Protein post-translational modifications|| |
Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events. / Phosphorylation on Ser-387 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes.
|Protein cellular localization|| |
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St John’s Laboratory Ltd.
|Product type|| |
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