Mouse Monoclonal CD4 antibody [11A1] (STJ96972)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal CD4 antibody [11A1] (STJ96972)

Supplier: St John’s Laboratory Ltd.

Recommended applications: IHC

Recommended dilution: IHC 1:200

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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CD4 antibody,|CD 4 antibody|CD4 (L3T4) antibody|CD4 antibody|CD4 antigen (p55) antibody|CD4 antigen antibody|CD4 molecule antibody|CD4 receptor antibody|CD4+ Lymphocyte deficiency, included antibody|CD4_HUMAN antibody|CD4mut antibody|L3T4 antibody|Leu3 antibody|Ly-4 antibody|Lymphocyte antigen CD4 antibody|MGC165891 antibody|OTTHUMP00000238897 antibody|p55 antibody|T cell antigen T4 antibody|T cell antigen T4/LEU3 antibody|T cell differentiation antigen L3T4 antibody|T cell OKT4 deficiency, included antibody|T cell surface antigen T4/Leu 3 antibody|T cell surface antigen T4/Leu3 antibody|T cell surface glycoprotein CD4 antibody|T-cell surface antigen T4/Leu-3 antibody|T-cell surface glycoprotein CD4 antibody|W3/25 antibody|W3/25 antigen antibody|Anti-CD4 antibody [EPR6855] (ab133616)
SCBT cat No: sc-70660|sc-59031|sc-1176|sc-59032|sc-70674|sc-70670|sc-70659|sc-514571|sc-32811|sc-59034|sc-514746|sc-1140|sc-51544|sc-13573|sc-19642|sc-7219|sc-51545|sc-51547|sc-19641|sc-53042|sc-52385|sc-19643|

Name

CD4 Monoclonal Antibody

Catalogue No.

STJ96972

Reactivity

Human, Mouse, Rat

Specificity

The antibody detects endogenous CD4 proteins.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

IHC

Recommended dilution

IHC 1:200

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

CD4 Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

CD4 Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

T-cell surface glycoprotein CD4 antibody, T-cell surface antigen T4/Leu-3 antibody, CD antigen CD4 antibody

Database links

Human UniProt/Swiss-Prot:P01730;Mouse UniPort/Swiss-Prot: P06332;Rat UniProt/Swiss-Port: P05540;Human Entrez Gene: 920;Mouse Entrez Gene: 12504;Rat Entrez Gene: Rn.10748

Protein names

T-cell surface glycoprotein CD4 , T-cell surface antigen T4/Leu-3 , CD antigen CD4

Protein function

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. / (Microbial infection) Acts as a receptor for human immunodeficiency virus-1 . Down-regulated by HIV-1 Vpu . Acts as a receptor for Human Herpes virus 7/HHV-7 .

Protein sequence and domain

Contains 3 Ig-like C2-type (immunoglobulin-like) domains. / Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Protein post-translational modifications

Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

Protein cellular localization

Cell membrane / Single-pass type I membrane protein

Research area

All research areas>Lymphocyte Signaling>CD4
(View all antibody categories related to Lymphocyte Signaling)

Note

AntibodyPlus can customize CD4 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Mouse colon tissue

1: CD4 Monoclonal Antibody(11A1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse colon tissue

1: CD4 Monoclonal Antibody(11A1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse colon tissue

1: CD4 Monoclonal Antibody(11A1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat lung tissue

1: CD4 Monoclonal Antibody(11A1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat lung tissue

1: CD4 Monoclonal Antibody(11A1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat lung tissue

1: CD4 Monoclonal Antibody(11A1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat testis tissue

1: CD4 Monoclonal Antibody(11A1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat testis tissue

1: CD4 Monoclonal Antibody(11A1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human stomach tissue

1: CD4 Monoclonal Antibody(11A1) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: CD4 Monoclonal Antibody(11A1) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: CD4 Monoclonal Antibody(11A1) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat brain tissue

1: CD4 Monoclonal Antibody(11A1) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse brain tissue

1: CD4 Monoclonal Antibody(11A1) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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