Mouse Monoclonal CD45 antibody [12A9] (STJ96948)

$99.00$319.00

Reactivity: Human
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal CD45 antibody [12A9] (STJ96948)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB

Recommended dilution: WB 1:2000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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PTPRC antibody, CD45 antibody,|B220 antibody|CD45 antibody|cd45 antigen antibody|CD45R antibody|GP180 antibody|L-CA antibody|lca antibody|Leukocyte common antigen antibody|LY 5 antibody|LY5 antibody|Protein tyrosine phosphatase, receptor type, C antibody|Protein tyrosine phosphatase, receptor type, c polypeptide antibody|Ptprc antibody|PTPRC_HUMAN antibody|Receptor-type tyrosine-protein phosphatase C antibody|T200 antibody|T200 glycoprotein antibody|T200 leukocyte common antigen antibody|Anti-CD45 antibody (ab10558)
SCBT cat No: sc-53665|sc-53201|sc-1178|sc-70699|sc-70700|sc-28369|sc-52490|sc-25590|sc-52491|

Name

CD45 Monoclonal Antibody

Catalogue No.

STJ96948

Reactivity

Human

Specificity

The antibody detects endogenous CD45 proteins.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

WB

Recommended dilution

WB 1:2000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

CD45 Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

CD45 Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Receptor-type tyrosine-protein phosphatase C antibody, Leukocyte common antigen antibody, L-CA antibody, T200 antibody, CD antigen CD45 antibody

Database links

Human UniProt/Swiss-Prot:P08575;Rat UniProt/Swiss-Port: P04157;Human Entrez Gene: 5788;Mouse Entrez Gene: 19264;Rat Entrez Gene: Rn.90166

Protein names

Receptor-type tyrosine-protein phosphatase C , Leukocyte common antigen , L-CA , T200 , CD antigen CD45

Protein function

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). / Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Involvement in disease

Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]: A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Multiple sclerosis (MS) [MIM:126200]: A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheath, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. . Note: Disease susceptibility may be associated with variations affecting the gene represented in this entry.

Protein sequence and domain

The first PTPase domain interacts with SKAP1. / Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. / Contains 2 fibronectin type-III domains. / Contains 2 tyrosine-protein phosphatase domains.

Protein post-translational modifications

Heavily N- and O-glycosylated.

Protein cellular localization

Membrane / Single-pass type I membrane protein / Membrane raft

Research area

All research areas>Lymphocyte Signaling>CD45
(View all antibody categories related to Lymphocyte Signaling)

Note

AntibodyPlus can customize CD45 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human liver cancer tissue

1: CD45 Monoclonal Antibody(12A9)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: CD45 Monoclonal Antibody(12A9)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung cancer tissue

1: CD45 Monoclonal Antibody(12A9)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung cancer tissue

1: CD45 Monoclonal Antibody(12A9)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human kidney cancer tissue

1: CD45 Monoclonal Antibody(12A9)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human kidney cancer tissue

1: CD45 Monoclonal Antibody(12A9)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue

1: CD45 Monoclonal Antibody(12A9) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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