Mouse Monoclonal CD5 antibody [10G8] (STJ96973)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal CD5 antibody [10G8] (STJ96973)

Supplier: St John’s Laboratory Ltd.

Recommended applications: IHC

Recommended dilution: IHC 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for CD5 Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

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CD5 antibody, LEU1 antibody,|CD 5 antibody|CD5 antibody|CD5 antigen (p56 62) antibody|CD5 antigen antibody|CD5 molecule antibody|CD5_HUMAN antibody|LEU1 antibody|LyA antibody|Lymphocyte antigen T1/Leu-1 antibody|OTTHUMP00000236973 antibody|T-cell surface glycoprotein CD5 antibody|T1 antibody|Anti-CD5 antibody [EP2952] (ab75877)
SCBT cat No: sc-53204|sc-393019|sc-393087|sc-20920|sc-53205|sc-69734|sc-18898|sc-6986|sc-1180|

Name

CD5 Monoclonal Antibody

Catalogue No.

STJ96973

Reactivity

Human, Mouse, Rat

Specificity

The antibody detects endogenous CD5 proteins.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

IHC

Recommended dilution

IHC 1:200

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

CD5 Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

CD5 Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

T-cell surface glycoprotein CD5 antibody, Lymphocyte antigen T1/Leu-1 antibody, CD antigen CD5 antibody

Database links

Human UniProt/Swiss-Prot:P06127;Mouse UniPort/Swiss-Prot: P13379;Rat UniProt/Swiss-Port: P51882;Human Entrez Gene: 921;Mouse Entrez Gene: 12507;Rat Entrez Gene: Rn.11337

Protein names

T-cell surface glycoprotein CD5 , Lymphocyte antigen T1/Leu-1 , CD antigen CD5

Protein function

May act as a receptor in regulating T-cell proliferation.

Protein sequence and domain

Contains 3 SRCR domains.

Protein post-translational modifications

Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1.

Protein cellular localization

Cell membrane; Single-pass type I membrane protein

Research area

All research areas>Lymphocyte Signaling>CD5
(View all antibody categories related to Lymphocyte Signaling)

Note

AntibodyPlus can customize CD5 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Mouse heart tissue

1: CD5 Monoclonal Antibody(10G8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse heart tissue

1: CD5 Monoclonal Antibody(10G8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse heart tissue

1: CD5 Monoclonal Antibody(10G8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: CD5 Monoclonal Antibody(10G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach tissue

1: CD5 Monoclonal Antibody(10G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: CD5 Monoclonal Antibody(10G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: CD5 Monoclonal Antibody(10G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat spinal cord tissue

1: CD5 Monoclonal Antibody(10G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat brain tissue

1: CD5 Monoclonal Antibody(10G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat spleen tissue

1: CD5 Monoclonal Antibody(10G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue

1: CD5 Monoclonal Antibody(10G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse brain tissue

1: CD5 Monoclonal Antibody(10G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse spleen tissue

1: CD5 Monoclonal Antibody(10G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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