Mouse Monoclonal CD68 antibody [6F3] (STJ96952)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal CD68 antibody [6F3] (STJ96952)

Supplier: St John’s Laboratory Ltd.

Recommended applications: IHC

Recommended dilution: IHC 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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CD68 antibody,|CD 68 antibody|CD68 antibody|CD68 antigen antibody|CD68 molecule antibody|CD68_HUMAN antibody|DKFZp686M18236 antibody|gp11 antibody|Gp110 antibody|LAMP4 antibody|Macrophage antigen CD68 (microsialin) antibody|MACROPHAGE ANTIGEN CD68 antibody|Macrosialin antibody|SCARD1 antibody|Scavenger receptor class D member 1 antibody|Anti-CD68 antibody [KP1] (ab955)
SCBT cat No: sc-7084|sc-7083|

Name

CD68 Monoclonal Antibody

Catalogue No.

STJ96952

Reactivity

Human, Mouse, Rat

Specificity

The antibody detects endogenous CD68 proteins.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

IHC

Recommended dilution

IHC 1:200

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

CD68 Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

CD68 Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Macrosialin antibody, Gp110 antibody, CD antigen CD68 antibody

Database links

Human UniProt/Swiss-Prot:P34810;Mouse UniPort/Swiss-Prot: P31996;Rat UniProt/Swiss-Port: Q4FZY1;Human Entrez Gene: 968;Mouse Entrez Gene: 12514;Rat Entrez Gene: Rn.12478

Protein names

Macrosialin , Gp110 , CD antigen CD68

Protein function

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

Protein tissue specificity

Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.

Protein sequence and domain

Belongs to the LAMP family.

Protein post-translational modifications

N- and O-glycosylated.

Protein cellular localization

Cell membrane; Single-pass type I membrane protein / Endosome membrane; Single-pass type I membrane protein / Lysosome membrane; Single-pass type I membrane protein

Research area

All research areas>Lymphocyte Signaling>CD68
(View all antibody categories related to Lymphocyte Signaling)

Note

AntibodyPlus can customize CD68 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human lung cancer tissue

1: CD68 Monoclonal Antibody(6F3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung cancer tissue

1: CD68 Monoclonal Antibody(6F3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: CD68 Monoclonal Antibody(6F3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: CD68 Monoclonal Antibody(6F3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human Tonsil tissue

1: CD68 Monoclonal Antibody(6F3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human colon tissue

1: CD68 Monoclonal Antibody(6F3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach tissue

1: CD68 Monoclonal Antibody(6F3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse liver tissue

1: CD68 Monoclonal Antibody(6F3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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