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Mouse Monoclonal Cdk4 antibody (STJ98476)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB
Recommended dilution: WB 1:1000-1:2000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
CDK4 antibody,|Cdk 4 antibody|cdk4 antibody|CDK4 protein antibody|CDK4_HUMAN antibody|Cell division kinase 4 antibody|Cell division protein kinase 4 antibody|CMM 3 antibody|CMM3 antibody|Crk3 antibody|Cyclin dependent kinase 4 antibody|Cyclin-dependent kinase 4 antibody|Melanoma cutaneous malignant 3 antibody|MGC14458 antibody|p34 cdk4 antibody|PSK J3 antibody|PSK-J3 antibody|Anti-Cdk4 antibody [EPR4513] (ab108357) KO Validated
SCBT cat No: sc-70832|sc-70831|sc-136241|sc-166373|sc-260|sc-53636|sc-56277|sc-23896|sc-393653|sc-601|sc-749|sc-56361|
Cdk4 Monoclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat, Dog, Pig
Cdk4 Monoclonal Antibody detects endogenous levels of Cdk4 protein.
Purified recombinant human Cdk4 protein fragments expressed in Ecoli
|Recommended dilution|| |
Cdk4 Antibody was tube-contained. Purified in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Cdk4 Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Cyclin-dependent kinase 4 antibody, Cell division protein kinase 4 antibody, PSK-J3 antibody
|Protein names|| |
Cyclin-dependent kinase 4 , Cell division protein kinase 4 , PSK-J3
|Protein function|| |
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. / ATP + a protein = ADP + a phosphoprotein. / Both phosphorylation at Thr-172 and binding of a D-type cyclin are necessary for enzymatic activity. Full activation of the cyclin-D-CDK4 complex appears to require other factors such as recruitment of the substrate via a substrate recruitment motif, and/or formation of the CDKN1B ternary complex. Inhibited by INK4 family members. In resting cells, the non-tyrosine-phosphorylated form of CDKN1B prevents phosphorylation at Thr-172 and inactivation, while, in proliferating cells, tyrosine phosphorylation of CDKN1B allows phosphorylation of Thr-172 of CDK4 and subsequennt activation.
|Involvement in disease|| |
Melanoma, cutaneous malignant 3 (CMM3) [MIM:609048]: A malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but also may involve other sites. . Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
|Protein cellular localization|| |
Cytoplasm / Nucleus / Membrane
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St John’s Laboratory Ltd.
|Product type|| |
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