Mouse Monoclonal CHOP antibody [2B1] (STJ97774)

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Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal CHOP antibody [2B1] (STJ97774)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC

Recommended dilution: WB 1:1000-2000; IHC 1:100-200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for CHOP Mouse Monoclonal Antibody(2B1).

Alternative names:

Check alternative names for the antibody

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DDIT3 antibody, CHOP antibody, CHOP10 antibody, GADD153 antibody,|C/EBP homologous protein antibody|C/EBP Homology Protein antibody|C/EBP zeta antibody|C/EBP-homologous protein 10 antibody|C/EBP-homologous protein antibody|CCAAT/enhancer binding protein homologous protein antibody|CEBPZ antibody|CHOP 10 antibody|CHOP antibody|CHOP-10 antibody|CHOP10 antibody|DDIT 3 antibody|DDIT-3 antibody|Ddit3 antibody|DDIT3_HUMAN antibody|DNA Damage Inducible Transcript 3 antibody|DNA damage-inducible transcript 3 protein antibody|GADD 153 antibody|GADD153 antibody|Growth Arrest and DNA Damage Inducible Protein 153 antibody|Growth arrest and DNA damage inducible protein GADD153 antibody|Growth arrest and DNA damage-inducible protein GADD153 antibody|MGC4154 antibody|Anti-DDIT3 antibody [9C8] (ab11419)
SCBT cat No: sc-71136|sc-56107|sc-7351|sc-575|sc-166682|sc-793|

Name

CHOP Mouse Monoclonal Antibody(2B1)

Catalogue No.

STJ97774

Reactivity

Human, Mouse, Rat

Specificity

CHOP Mouse Monoclonal Antibody(2B1) detects endogenous levels of DDIT3

Immunogen

Synthetic Peptide of CHOP at AA range of 10-90

Host

Mouse

Applications

WB, IHC

Recommended dilution

WB 1:1000-2000; IHC 1:100-200

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

CHOP Antibody(2B1) was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

CHOP Mouse Antibody(2B1) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

DNA damage-inducible transcript 3 protein antibody, DDIT-3 antibody, C/EBP zeta antibody, C/EBP-homologous protein antibody, CHOP antibody, C/EBP-homologous protein 10 antibody, CHOP-10 antibody, CCAAT/enhancer-binding protein homologous protein antibody, Growth arrest and DNA damage-inducible protein GADD153 antibody

Database links

Human UniProt/Swiss-Prot:P35638;Mouse UniPort/Swiss-Prot: P35639;Rat UniProt/Swiss-Port: Q496Y7;Human Entrez Gene: 1649;Mouse Entrez Gene: 13198;Rat Entrez Gene: Rn.11183

Protein names

DNA damage-inducible transcript 3 protein , DDIT-3 , C/EBP zeta , C/EBP-homologous protein , CHOP , C/EBP-homologous protein 10 , CHOP-10 , CCAAT/enhancer-binding protein homologous protein , Growth arrest and DNA damage-inducible protein GADD153

Protein function

Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response.

Protein tissue specificity

By oxidative stress, amino-acid deprivation, hypoxia and ER stress. During ER stress, induced by a EIF2AK3/ATF4 pathway and/or ERN1/ATF6 pathway. Expression is suppressed by TLR-TRIF signaling pathway during prolonged ER stress.

Involvement in disease

Myxoid liposarcoma (MXLIPO) [MIM:613488]: A soft tissue tumor that tends to occur in the limbs (especially the thigh) of patients ranging in age from 35 to 55 years. It is defined by the presence of a hypocellular spindle cell proliferation set in a myxoid background, often with mucin pooling. Lipoblasts tend to be small and often monovacuolated and to cluster around vessels or at the periphery of the lesion. . Note: The gene represented in this entry may be involved in disease pathogenesis. A chromosomal aberration involving DDIT3 has been found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with FUS (PubMed:7503811). .

Protein sequence and domain

The N-terminal region is necessary for its proteasomal degradation, transcriptional activity and interaction with EP300/P300. / Belongs to the bZIP family. / Contains 1 bZIP (basic-leucine zipper) domain.

Protein post-translational modifications

Ubiquitinated, leading to its degradation by the proteasome. / Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity.

Protein cellular localization

Cytoplasm / Nucleus

Research area

All research areas>Tumor Suppressor/Apoptosis>GADD
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize CHOP Antibody(2B1) according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Mouse brain tissue

1: CHOP Mouse Monoclonal Antibody(2B1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse brain tissue

1: CHOP Mouse Monoclonal Antibody(2B1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human stomach tissue

1: CHOP Mouse Monoclonal Antibody(2B1) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue

1: CHOP Mouse Monoclonal Antibody(2B1) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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