Mouse Monoclonal CK16 antibody [6F6] (STJ96958)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal CK16 antibody [6F6] (STJ96958)

Supplier: St John’s Laboratory Ltd.

Recommended applications: IHC

Recommended dilution: IHC 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for CK16 Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

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KRT16 antibody, KRT16A antibody,|CK 16 antibody|CK-16 antibody|CK16 antibody|Cytokeratin-16 antibody|Cytokeratin16 antibody|FNEPPK antibody|Focal non epidermolytic palmoplantar keratoderma antibody|K 16 antibody|K16 antibody|K1C16_HUMAN antibody|K1CP antibody|Keratin 1 type I antibody|Keratin 16 antibody|Keratin antibody|Keratin type I cytoskeletal 16 antibody|Keratin-16 antibody|Keratin16 antibody|KRT 16 antibody|Krt16 antibody|KRT16A antibody|NEPPK antibody|PC1 antibody|type I cytoskeletal 16 antibody|Anti-Cytokeratin 16 antibody [EP1615Y] (ab76416)
SCBT cat No: sc-377224|sc-271937|sc-53255|

Name

CK16 Monoclonal Antibody

Catalogue No.

STJ96958

Reactivity

Human, Mouse, Rat

Specificity

The antibody detects endogenous CK16 proteins.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

IHC

Recommended dilution

IHC 1:200

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

CK16 Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

CK16 Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Keratin, type I cytoskeletal 16 antibody, Cytokeratin-16 antibody, CK-16 antibody, Keratin-16 antibody, K16 antibody

Database links

Human UniProt/Swiss-Prot:P08779;Mouse UniPort/Swiss-Prot: Q9Z2K1;Rat UniProt/Swiss-Port: Q6IFU9;Human Entrez Gene: 3868;Mouse Entrez Gene: 16666;Rat Entrez Gene: Rn.145931

Protein names

Keratin, type I cytoskeletal 16 , Cytokeratin-16 , CK-16 , Keratin-16 , K16

Protein function

Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.

Protein tissue specificity

Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.

Involvement in disease

Pachyonychia congenita 1 (PC1) [MIM:167200]: An autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma, follicular hyperkeratosis, and oral leukokeratosis. Hyperhidrosis of the hands and feet is usually present. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Keratoderma, palmoplantar, non-epidermolytic, focal 1 (FNEPPK1) [MIM:613000]: A dermatological disorder characterized by non-epidermolytic palmoplantar keratoderma limited to the pressure points on the balls of the feet, with later mild involvement on the palms. Oral, genital and follicular keratotic lesions are often present. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Unilateral palmoplantar verrucous nevus (UPVN) [MIM:144200]: UPVN is characterized by a localized epidermolytic hyperkeratosis in parts of the right palm and the right sole, following the lines of Blaschko. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Note: KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.

Protein sequence and domain

Belongs to the intermediate filament family.

Research area

All research areas>Structural Proteins>Cytokeratin 16
(View all antibody categories related to Structural Proteins)

Note

AntibodyPlus can customize CK16 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human liver cancer tissue

1: CK16 Monoclonal Antibody(6F6)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: CK16 Monoclonal Antibody(6F6)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: CK16 Monoclonal Antibody(6F6)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse lung tissue

1: CK16 Monoclonal Antibody(6F6)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse lung tissue

1: CK16 Monoclonal Antibody(6F6)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse lung tissue

1: CK16 Monoclonal Antibody(6F6)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat liver tissue

1: CK16 Monoclonal Antibody(6F6)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat liver tissue

1: CK16 Monoclonal Antibody(6F6)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat liver tissue

1: CK16 Monoclonal Antibody(6F6)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat lung tissue

1: CK16 Monoclonal Antibody(6F6)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat lung tissue

1: CK16 Monoclonal Antibody(6F6)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat lung tissue

1: CK16 Monoclonal Antibody(6F6)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human Tonsil tissue

1: CK16 Monoclonal Antibody(6F6) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human colon tissue

1: CK16 Monoclonal Antibody(6F6) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver tissue

1: CK16 Monoclonal Antibody(6F6) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: CK16 Monoclonal Antibody(6F6) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung tissue

1: CK16 Monoclonal Antibody(6F6) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue

1: CK16 Monoclonal Antibody(6F6) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach tissue

1: CK16 Monoclonal Antibody(6F6) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: CK16 Monoclonal Antibody(6F6) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat lung tissue

1: CK16 Monoclonal Antibody(6F6) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: CK16 Monoclonal Antibody(6F6) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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