Mouse Monoclonal CK8 antibody [8G8] (STJ96957)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal CK8 antibody [8G8] (STJ96957)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC

Recommended dilution: WB 1:2000-5000; IHC 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for CK8 Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

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KRT8 antibody, CYK8 antibody,|CARD2 antibody|Cell proliferation inducing gene 46 protein antibody|Cell proliferation inducing protein 46 antibody|CK 8 antibody|CK-8 antibody|CK18 antibody|CK8 antibody|CYK18 antibody|CYK8 antibody|Cytokeratin 18 antibody|Cytokeratin 8 antibody|Cytokeratin-8 antibody|K18 antibody|K2C8 antibody|K2C8_HUMAN antibody|K8 antibody|Keratin 18 antibody|Keratin 8 antibody|Keratin antibody|Keratin type I cytoskeletal 18 antibody|keratin type II cytoskeletal 8 antibody|Keratin-8 antibody|KO antibody|KRT18 antibody|KRT8 antibody|type II cytoskeletal 8 antibody|Type-II keratin Kb8 antibody|Anti-Cytokeratin 8+18 antibody [EP1628Y] (ab53280)
SCBT cat No: sc-130312|sc-58736|sc-73480|sc-58737|sc-374275|sc-8020|sc-374274|sc-374027|sc-134484|sc-101459|sc-53266|sc-52324|

Name

CK8 Monoclonal Antibody

Catalogue No.

STJ96957

Reactivity

Human, Mouse, Rat

Specificity

The antibody detects endogenous CK8 proteins.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

WB, IHC

Recommended dilution

WB 1:2000-5000; IHC 1:200

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

CK8 Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

CK8 Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Keratin, type II cytoskeletal 8 antibody, Cytokeratin-8 antibody, CK-8 antibody, Keratin-8 antibody, K8 antibody, Type-II keratin Kb8 antibody

Database links

Human UniProt/Swiss-Prot:P05787;Mouse UniPort/Swiss-Prot: P11679;Rat UniProt/Swiss-Port: Q10758;Human Entrez Gene: 3856;Mouse Entrez Gene: 16691;Rat Entrez Gene: Rn.11083

Protein names

Keratin, type II cytoskeletal 8 , Cytokeratin-8 , CK-8 , Keratin-8 , K8 , Type-II keratin Kb8

Protein function

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Protein tissue specificity

Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

Involvement in disease

Cirrhosis (CIRRH) [MIM:215600]: A liver disease characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper. Clinical features include abdomen swelling, jaundice and pulmonary hypertension. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the intermediate filament family.

Protein post-translational modifications

Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. / O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. / O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Protein cellular localization

Cytoplasm / Nucleus > nucleoplasm / Nucleus matrix

Note

AntibodyPlus can customize CK8 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human liver cancer tissue

1: CK8 Monoclonal Antibody(8G8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: CK8 Monoclonal Antibody(8G8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat liver tissue

1: CK8 Monoclonal Antibody(8G8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat liver tissue

1: CK8 Monoclonal Antibody(8G8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse lung tissue

1: CK8 Monoclonal Antibody(8G8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse lung tissue

1: CK8 Monoclonal Antibody(8G8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human colon tissue

1: CK8 Monoclonal Antibody(8G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: CK8 Monoclonal Antibody(8G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach tissue

1: CK8 Monoclonal Antibody(8G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: CK8 Monoclonal Antibody(8G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat liver tissue

1: CK8 Monoclonal Antibody(8G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat lung tissue

1: CK8 Monoclonal Antibody(8G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: CK8 Monoclonal Antibody(8G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse liver tissue

1: CK8 Monoclonal Antibody(8G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse lung tissue

1: CK8 Monoclonal Antibody(8G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue

1: CK8 Monoclonal Antibody(8G8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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