Mouse Monoclonal Crk II antibody (STJ98480)

$379.00

Reactivity: Human, Mouse, Dog, Rabbit
Applications: WB
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Mouse Monoclonal Crk II antibody (STJ98480)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB

Recommended dilution: WB 1:1000-1:2000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Crk II Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

Expand

CRK antibody,|Adapter molecule crk antibody|Avian sarcoma virus CT10 (v crk) oncogene homolog antibody|CRK antibody|CRK isoform 2 antibody|CRK isoform II antibody|CRK_HUMAN antibody|CRKII antibody|FLJ38130 antibody|OTTHUMP00000115366 antibody|OTTHUMP00000198330 antibody|p38 antibody|Proto oncogene C crk antibody|Proto-oncogene C-crk antibody|v crk avian sarcoma virus CT10 oncogene homolog antibody|v crk sarcoma virus CT10 oncogene homolog antibody|v crk sarcoma virus CT10 oncogene homolog (avian) antibody|Anti-Crk p38 antibody [EPR4984(2)] (ab133581)
SCBT cat No: sc-393160|sc-23775|sc-17989|sc-81234|sc-390132|sc-289|sc-9004|

 

Name

Crk II Monoclonal Antibody

Catalogue No.

STJ98480

Reactivity

Human, Mouse, Dog, Rabbit

Specificity

Crk II Monoclonal Antibody detects endogenous levels of Crk II protein.

Immunogen

Purified recombinant human Crk II protein fragments expressed in Ecoli

Host

Mouse

Applications

WB

Recommended dilution

WB 1:1000-1:2000

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

Crk II Antibody was tube-contained. Purified in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.

Concentration

1 mg/ml

Purification

Crk II Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Adapter molecule crk antibody, Proto-oncogene c-Crk antibody, p38 antibody

Database links

Human UniProt/Swiss-Prot:P46108;Mouse UniPort/Swiss-Prot: Q64010;Rat UniProt/Swiss-Port: Q63768;Human Entrez Gene: 1398;Mouse Entrez Gene: 12928;Rat Entrez Gene: Rn.96136

Protein names

Adapter molecule crk , Proto-oncogene c-Crk , p38

Protein function

The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.

Protein sequence and domain

The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation. / The SH2 domain mediates interaction with tyrosine phosphorylated proteins. Mediates interaction with SHB. / Belongs to the CRK family. / Contains 1 SH2 domain. / Contains 2 SH3 domains.

Protein post-translational modifications

Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form. Isoform Crk-II is phosphorylated by KIT. / Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway. / Proline isomerization at Pro-237 by PPIA acts as a switch between two conformations: an autoinhibitory conformation in the cis form, where the tandem SH3 domains interact intramolecularly, and an activated conformation in the trans form.

Protein cellular localization

Cytoplasm / Cell membrane

Research area

All research areas>Signaling Intermediates>Crk
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize Crk II Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!