Mouse Monoclonal CTCF antibody [1D11] (STJ97975)

$379.00

Reactivity: Human, Monkey
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal CTCF antibody [1D11] (STJ97975)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:10000

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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CTCF antibody,|11 zinc finger protein antibody|11 zinc finger transcriptional repressor antibody|11-zinc finger protein antibody|CCCTC binding factor (zinc finger protein) antibody|CCCTC binding factor antibody|CCCTC-binding factor antibody|Ctcf antibody|CTCF_HUMAN antibody|CTCFL paralog antibody|MRD21 antibody|Transcriptional repressor CTCF antibody|Anti-CTCF antibody – ChIP Grade (ab70303)
SCBT cat No: sc-136284|sc-271514|sc-15914|sc-398149|sc-15913|sc-271474|sc-28198|sc-5916|

 

Name

CTCF Monoclonal Antibody

Catalogue No.

STJ97975

Reactivity

Human, Monkey

Specificity

CTCF Monoclonal Antibody detects endogenous levels of CTCF protein.

Immunogen

Purified recombinant fragment of human CTCF expressed in E Coli

Host

Mouse

Applications

WB, ELISA

Recommended dilution

WB 1:500-1:2000; ELISA 1:10000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

CTCF Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.

Concentration

1 mg/ml

Purification

CTCF Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Transcriptional repressor CTCF antibody, 11-zinc finger protein antibody, CCCTC-binding factor antibody, CTCFL paralog antibody

Database links

Human UniProt/Swiss-Prot:P49711;Mouse UniPort/Swiss-Prot: Q61164;Rat UniProt/Swiss-Port: Q9R1D1;Human Entrez Gene: 10664;Mouse Entrez Gene: 13018;Rat Entrez Gene: Rn.18612

Protein names

Transcriptional repressor CTCF , 11-zinc finger protein , CCCTC-binding factor , CTCFL paralog

Protein function

Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19.

Protein tissue specificity

Ubiquitous. Absent in primary spermatocytes.

Involvement in disease

Mental retardation, autosomal dominant 21 (MRD21) [MIM:615502]: A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. Additional MRD21 features include short stature, microcephaly, and developmental delay. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

The 11 zinc fingers are highly conserved among vertebrates, exhibiting almost identical amino acid sequences. Different subsets or combination of individual zinc fingers gives the ability to CTCF to recognize multiple DNA target sites. / Belongs to the CTCF zinc-finger protein family. / Contains 11 C2H2-type zinc fingers.

Protein post-translational modifications

Sumoylated on Lys-74 and Lys-689; sumoylation of CTCF contributes to the repressive function of CTCF on the MYC P2 promoter.

Protein cellular localization

Nucleus > nucleoplasm / Chromosome / Chromosome > centromere

Research area

All research areas>Transcription Regulators>CTCF
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize CTCF Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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