Mouse Monoclonal CYCS antibody [4B10] (STJ97419)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal CYCS antibody [4B10] (STJ97419)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC

Recommended dilution: WB 1:1000-5000; IHC 1:500-1000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for CYCS Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

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CYCS antibody, CYC antibody,|CYC antibody|CYC_HUMAN antibody|CYCS antibody|Cytochrome c antibody|Cytochrome c somatic antibody|HCS antibody|THC4 antibody|Anti-Cytochrome C antibody [7H8.2C12] (ab13575)
SCBT cat No: sc-65396|sc-13561|sc-13560|sc-13156|sc-8385|sc-7159|

Name

CYCS Monoclonal Antibody

Catalogue No.

STJ97419

Reactivity

Human, Mouse, Rat

Specificity

The antibody detects endogenous CYCS protein.

Immunogen

Recombinant Protein

Host

Mouse

Applications

WB, IHC

Recommended dilution

WB 1:1000-5000; IHC 1:500-1000

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

CYCS Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

CYCS Antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Cytochrome c antibody

Database links

Human UniProt/Swiss-Prot:P99999;Mouse UniPort/Swiss-Prot: P62897;Rat UniProt/Swiss-Port: P62898;Human Entrez Gene: 54205;Mouse Entrez Gene: 13063;Rat Entrez Gene: Rn.161841

Protein names

Cytochrome c

Protein function

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. / Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

Involvement in disease

Thrombocytopenia 4 (THC4) [MIM:612004]: Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the cytochrome c family.

Protein post-translational modifications

Binds 1 heme group per subunit. / Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.

Protein cellular localization

Mitochondrion intermembrane space

Research area

All research areas>Tumor Suppressor/Apoptosis>Cytochrome
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize CYCS Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human liver cancer tissue

1: CYCS Monoclonal Antibody(4B10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: CYCS Monoclonal Antibody(4B10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: CYCS Monoclonal Antibody(4B10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: CYCS Monoclonal Antibody(4B10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat liver tissue

1: CYCS Monoclonal Antibody(4B10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat liver tissue

1: CYCS Monoclonal Antibody(4B10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human Tonsil tissue

1: CYCS Monoclonal Antibody(4B10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: CYCS Monoclonal Antibody(4B10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue

1: CYCS Monoclonal Antibody(4B10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse spleen tissue

1: CYCS Monoclonal Antibody(4B10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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