Mouse Monoclonal Cystatin C antibody [7F11] (STJ97732)

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Reactivity: Human
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Cystatin C antibody [7F11] (STJ97732)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:1000-2000; IHC 1:100-200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Cystatin C Mouse Monoclonal Antibody(7F11).

Alternative names:

Check alternative names for the antibody

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CST3 antibody,|AD 8 antibody|AD8 antibody|Amyloid angiopathy and cerebral hemorrhage antibody|ARMD11 antibody|bA218C14.4 (cystatin C) antibody|bA218C14.4 antibody|Cst 3 antibody|Cst3 antibody|CST3 protein antibody|Cystatin 3 antibody|Cystatin-3 antibody|Cystatin-C antibody|Cystatin3 antibody|CystatinC antibody|CYTC_HUMAN antibody|Gamma trace antibody|Gamma-trace antibody|HCCAA antibody|MGC117328 antibody|Neuroendocrine basic polypeptide antibody|Post gamma globulin antibody|Post-gamma-globulin antibody|Anti-Cystatin C antibody [EPR4413] (ab109508)
SCBT cat No: sc-293159|sc-73878|sc-51857|sc-66100|sc-16989|sc-73879|

Name

Cystatin C Mouse Monoclonal Antibody(7F11)

Catalogue No.

STJ97732

Reactivity

Human

Specificity

Cystatin C Mouse Monoclonal Antibody(7F11) detects endogenous levels of CST3

Immunogen

Recombinant Protein of Cystatin C

Host

Mouse

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:1000-2000; IHC 1:100-200

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

Cystatin C Antibody(7F11) was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Cystatin C Mouse Antibody(7F11) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Cystatin-C antibody, Cystatin-3 antibody, Gamma-trace antibody, Neuroendocrine basic polypeptide antibody, Post-gamma-globulin antibody

Database links

Human UniProt/Swiss-Prot:P01034;Mouse UniPort/Swiss-Prot: P21460;Rat UniProt/Swiss-Port: P14841;Human Entrez Gene: 1471;Mouse Entrez Gene: 13010;Rat Entrez Gene: Rn.106351

Protein names

Cystatin-C , Cystatin-3 , Gamma-trace , Neuroendocrine basic polypeptide , Post-gamma-globulin

Protein function

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.

Protein tissue specificity

Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.

Involvement in disease

Amyloidosis 6 (AMYL6) [MIM:105150]: A hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Macular degeneration, age-related, 11 (ARMD11) [MIM:611953]: A form of age-related macular degeneration, a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. . Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the cystatin family.

Protein post-translational modifications

The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.

Protein cellular localization

Secreted

Research area

All research areas>Signaling Intermediates>Cystatin
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize Cystatin C Antibody(7F11) according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human liver cancer tissue

1: Cystatin C Mouse Monoclonal Antibody(7F11)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: Cystatin C Mouse Monoclonal Antibody(7F11)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung cancer tissue

1: Cystatin C Mouse Monoclonal Antibody(7F11)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung cancer tissue

1: Cystatin C Mouse Monoclonal Antibody(7F11)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human liver tissue

1: Cystatin C Mouse Monoclonal Antibody(7F11) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: Cystatin C Mouse Monoclonal Antibody(7F11) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue

1: Cystatin C Mouse Monoclonal Antibody(7F11) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human kidney tissue

1: Cystatin C Mouse Monoclonal Antibody(7F11) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: Cystatin C Mouse Monoclonal Antibody(7F11) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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