Mouse Monoclonal DNA-PKCS antibody [3H6] (STJ98003)

$379.00

Reactivity: Human
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal DNA-PKCS antibody [3H6] (STJ98003)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:10000

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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PRKDC antibody, HYRC antibody, HYRC1 antibody,|DNA dependent protein kinase catalytic subunit antibody|DNA PK catalytic subunit antibody|DNA-dependent protein kinase catalytic subunit antibody|DNA-PK catalytic subunit antibody|DNA-PKcs antibody|DNAPK antibody|DNAPK catalytic subunit antibody|DNPK 1 antibody|DNPK1 antibody|Hyper radiosensitivity of murine scid mutation, complementing 1 antibody|HYRC 1 antibody|HYRC antibody|HYRC1 antibody|IMD26 antibody|p350 antibody|p460 antibody|PKRDC antibody|PRKDC antibody|PRKDC_HUMAN antibody|Protein Kinase DNA Activated Catalytic Polypeptide antibody|XRCC 7 antibody|XRCC7 antibody|Anti-DNA PKcs antibody [18-2] (ab1832)
SCBT cat No: sc-390698|sc-390849|sc-5282|sc-376207|

 

Name

DNA-PKCS Monoclonal Antibody

Catalogue No.

STJ98003

Reactivity

Human

Specificity

DNA-PKCS Monoclonal Antibody detects endogenous levels of DNA-PKCS protein.

Immunogen

Purified recombinant fragment of human DNA-PKCS expressed in E Coli

Host

Mouse

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:10000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

DNA-PKCS Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.

Concentration

1 mg/ml

Purification

DNA-PKCS Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

DNA-dependent protein kinase catalytic subunit antibody, DNA-PK catalytic subunit antibody, DNA-PKcs antibody, DNPK1 antibody, p460 antibody

Database links

Human UniProt/Swiss-Prot:P78527;Mouse UniPort/Swiss-Prot: P97313;Rat UniProt/Swiss-Port: D3ZTN0;Human Entrez Gene: 5591;Mouse Entrez Gene: 19090;

Protein names

DNA-dependent protein kinase catalytic subunit , DNA-PK catalytic subunit , DNA-PKcs , DNPK1 , p460

Protein function

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates ‘Ser-139’ of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 ‘Ser-588’ and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation. / ATP + a protein = ADP + a phosphoprotein. / Inhibited by wortmannin. Activity of the enzyme seems to be attenuated by autophosphorylation.

Involvement in disease

Immunodeficiency 26 with or without neurologic abnormalities (IMD26) [MIM:615966]: A form of severe combined immunodeficiency characterized by reduced or absent T and B cells, recurrent candidiasis, and lower respiratory tract infections. Some patients show dysmorphic features, severe growth failure, microcephaly, seizures, and impaired neurological functions. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the PI3/PI4-kinase family. / Contains 1 FAT domain. / Contains 1 FATC domain. / Contains 2 HEAT repeats. / Contains 1 PI3K/PI4K domain. / Contains 3 TPR repeats.

Protein post-translational modifications

Autophosphorylated on Ser-2056, Thr-2609, Thr-2638 and Thr-2647. Ser-2056 and Thr-2609 are DNA damage-inducible phosphorylation sites (inducible with ionizing radiation, IR) dephosphorylated by PPP5C. Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair. / S-nitrosylated by GAPDH. / Polyubiquitinated by RNF144A, leading to proteasomal degradation.

Protein cellular localization

Nucleus / Nucleus > nucleolus

Research area

All research areas>Tumor Suppressor/Apoptosis>DNA-PK
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize DNA-PKCS Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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