Mouse Monoclonal EF-2 antibody [5B6] (STJ98011)

$379.00

Reactivity: Human
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal EF-2 antibody [5B6] (STJ98011)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:10000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for EF-2 Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

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EEF2 antibody, EF2 antibody,|EEF 2 antibody|Eef2 antibody|EF-2 antibody|EF2 antibody|EF2_HUMAN antibody|Elongation factor 2 antibody|Eukaryotic translation elongation factor 2 antibody|Polypeptidyl tRNA translocase antibody|SCA26 antibody|Anti-EEF2 antibody (ab33523)
SCBT cat No: sc-13004|sc-166415|sc-166409|sc-25634|sc-390014|sc-13003|

 

Name

EF-2 Monoclonal Antibody

Catalogue No.

STJ98011

Reactivity

Human

Specificity

EF-2 Monoclonal Antibody detects endogenous levels of EF-2 protein.

Immunogen

Purified recombinant fragment of human EF-2 expressed in E Coli

Host

Mouse

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:10000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

EF-2 Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.

Concentration

1 mg/ml

Purification

EF-2 Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Elongation factor 2 antibody, EF-2 antibody

Database links

Human UniProt/Swiss-Prot:P13639;Mouse UniPort/Swiss-Prot: P58252;Rat UniProt/Swiss-Port: P05197;Human Entrez Gene: 1938;Mouse Entrez Gene: 13629;Rat Entrez Gene: Rn.55145

Protein names

Elongation factor 2 , EF-2

Protein function

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

Involvement in disease

Spinocerebellar ataxia 26 (SCA26) [MIM:609306]: A form of spinocerebellar ataxia, a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily. / Contains 1 tr-type G (guanine nucleotide-binding) domain.

Protein post-translational modifications

Phosphorylation by EF-2 kinase completely inactivates EF-2; it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment. / Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis (By similarity). / ISGylated. / Proteolytically processed at two sites following phosphorylation by CSK. / SUMOytated following phosphorylation by CSK, promotes proteolytic cleavage.

Protein cellular localization

Cytoplasm / Nucleus

Research area

All research areas>Transcription Regulators>EF-2
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize EF-2 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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