Mouse Monoclonal eIF4A1 antibody (STJ97038)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal eIF4A1 antibody (STJ97038)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IF

Recommended dilution: WB 1:1000-3000; IF 1:100-200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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EIF4A1 antibody, DDX2A antibody, EIF4A antibody,|ATP-dependent RNA helicase eIF4A-1 antibody|DDX2 antibody|DDX2A antibody|eIF 4A I antibody|eIF-4A-I antibody|EIF4A antibody|eIF4A I antibody|eIF4A-I antibody|EIF4A1 antibody|Eukaryotic initiation factor 4A-I antibody|Eukaryotic initiation factor 4AI antibody|eukaryotic translation initiation factor 4A, isoform 1 antibody|Eukaryotic translation initiation factor 4A1 antibody|IF4A1_HUMAN antibody|Anti-eIF4A1 antibody (ab31217)
SCBT cat No: sc-14211|

Name

eIF4A1 Monoclonal Antibody

Catalogue No.

STJ97038

Reactivity

Human, Mouse, Rat

Specificity

The antibody detects endogenous eIF4A1 protein.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

WB, IF

Recommended dilution

WB 1:1000-3000; IF 1:100-200

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

eIF4A1 Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

eIF4A1 Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Eukaryotic initiation factor 4A-I antibody, eIF-4A-I antibody, eIF4A-I antibody, ATP-dependent RNA helicase eIF4A-1 antibody

Database links

Human UniProt/Swiss-Prot:P60842;Mouse UniPort/Swiss-Prot: P60843;Rat UniProt/Swiss-Port: Q6P3V8;Human Entrez Gene: 1973;Mouse Entrez Gene: 13681;Rat Entrez Gene: Rn.91523

Protein names

Eukaryotic initiation factor 4A-I , eIF-4A-I , eIF4A-I , ATP-dependent RNA helicase eIF4A-1

Protein function

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5′-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. / ATP + H2O = ADP + phosphate. / Helicase activity and function in translation are inhibited by interaction with PDCD4.

Protein sequence and domain

Belongs to the DEAD box helicase family. eIF4A subfamily. / Contains 1 helicase ATP-binding domain. / Contains 1 helicase C-terminal domain.

Research area

All research areas>Signaling Intermediates>eIF
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize eIF4A1 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Mouse spleen tissue

1: eIF4A1 Monoclonal Antibody(M8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse testis tissue

1: eIF4A1 Monoclonal Antibody(M8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse testis tissue

1: eIF4A1 Monoclonal Antibody(M8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse testis tissue

1: eIF4A1 Monoclonal Antibody(M8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human colon cancer tissue

1: eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver tissue

1: eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat lung tissue

1: eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat spinal cord tissue

1: eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat spleen tissue

1: eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse colon tissue

1: eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue

1: eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse brain tissue

1: eIF4A1 Monoclonal Antibody(M8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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