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Mouse Monoclonal Emt antibody [5G12C4] (STJ98019)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:10000
Recommended protocols: check protocols
Click or hover above images to see image description for Emt Monoclonal Antibody.
Check alternative names for the antibodyExpand
ITK antibody, EMT antibody, LYK antibody,||
SCBT cat No: To be updated
Emt Monoclonal Antibody
|Catalogue No.|| |
Emt Monoclonal Antibody detects endogenous levels of Emt protein.
Purified recombinant fragment of Emt (aa2-110) expressed in E Coli
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:10000
Emt Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.
Emt Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Tyrosine-protein kinase ITK/TSK antibody, Interleukin-2-inducible T-cell kinase antibody, IL-2-inducible T-cell kinase antibody, Kinase EMT antibody, T-cell-specific kinase antibody, Tyrosine-protein kinase Lyk antibody
|Protein names|| |
Tyrosine-protein kinase ITK/TSK , Interleukin-2-inducible T-cell kinase , IL-2-inducible T-cell kinase , Kinase EMT , T-cell-specific kinase , Tyrosine-protein kinase Lyk
|Protein function|| |
Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / Zn2+
|Protein tissue specificity|| |
T-cell lines and natural killer cell lines.
|Involvement in disease|| |
Lymphoproliferative syndrome 1 (LPFS1) [MIM:613011]: A rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma. . Note: The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
The N-terminal PH domain allows ITK to be recruited to the plasma membrane by an activated PI3 kinase. This domain contains also a proline-rich region (PRR). The adjoining domain is a SH3 domain, which binds to PRR (from itself or from other proteins). Next, a SH2 domain is required for binding tyrosine-phosphorylated substrates. In the C-terminal region, the kinase domain is required for tyrosine phosphorylation. / Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily. / Contains 1 Btk-type zinc finger. / Contains 1 PH domain. / Contains 1 protein kinase domain. / Contains 1 SH2 domain. / Contains 1 SH3 domain.
|Protein post-translational modifications|| |
Phosphorylated at Tyr-512 in the activation loop of the kinase domain by LCK. Subsequent autophosphorylation at Tyr-180 leads to the kinase activation. The autophosphorylated Tyr-180 lies within the substrate binding sequence of the SH3 domain. / Ubiquitinated.
|Protein cellular localization|| |
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St John’s Laboratory Ltd.
|Product type|| |
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