Mouse Monoclonal Fibronectin antibody (STJ97039)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Antibody Validation Project, Antibody Trial Sample, Antibody Free Sample for your own antibody validation and submitting antibody reviews, AntibodyPlus

Mouse Monoclonal Fibronectin antibody (STJ97039)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB

Recommended dilution: WB 1:1000-2000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Fibronectin Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

Expand

FN1 antibody, FN antibody,|CIG antibody|Cold insoluble globulin antibody|Cold-insoluble globulin antibody|DKFZp686F10164 antibody|DKFZp686H0342 antibody|DKFZp686I1370 antibody|DKFZp686O13149 antibody|ED B antibody|Fibronectin 1 antibody|FINC antibody|FINC_HUMAN antibody|FN antibody|FN1 antibody|FNZ antibody|GFND antibody|GFND2 antibody|LETS antibody|Migration stimulating factor antibody|MSF antibody|Ugl-Y3 antibody|Anti-Fibronectin antibody (ab2413)
SCBT cat No: sc-69681|sc-52331|sc-81767|sc-81769|sc-271098|sc-6952|sc-73611|sc-8422|sc-53285|sc-53286|sc-9068|sc-59824|sc-59826|sc-6953|sc-18826|sc-18825|sc-18827|sc-69682|

Name

Fibronectin Monoclonal Antibody

Catalogue No.

STJ97039

Reactivity

Human, Mouse, Rat

Specificity

The antibody detects endogenous Fibronectin protein.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

WB

Recommended dilution

WB 1:1000-2000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

Fibronectin Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

Fibronectin Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Fibronectin antibody, FN antibody, Cold-insoluble globulin antibody, CIG antibody

Database links

Human UniProt/Swiss-Prot:P02751;Mouse UniPort/Swiss-Prot: P11276;Rat UniProt/Swiss-Port: A0A096P6L8;Human Entrez Gene: 2335;Mouse Entrez Gene: 14268;Rat Entrez Gene: Rn.1604

Protein names

Fibronectin , FN , Cold-insoluble globulin , CIG

Protein function

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. / Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.

Protein tissue specificity

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.

Involvement in disease

Glomerulopathy with fibronectin deposits 2 (GFND2) [MIM:601894]: Genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Contains 12 fibronectin type-I domains. / Contains 2 fibronectin type-II domains. / Contains 16 fibronectin type-III domains.

Protein post-translational modifications

Sulfated. / It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated. / Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). / Phosphorylated by FAM20C in the extracellular medium. / Proteolytic processing produces the C-terminal NC1 peptide, anastellin.

Protein cellular localization

Secreted > extracellular space > extracellular matrix

Research area

All research areas>Cell Adhesion Proteins>Fibronectin
(View all antibody categories related to Cell Adhesion Proteins)

Note

AntibodyPlus can customize Fibronectin Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews
 

Immunofluorescence analysis of Human appendix tissue

1: Fibronectin Monoclonal Antibody(M9)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human appendix tissue

1: Fibronectin Monoclonal Antibody(M9)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human appendix tissue

1: Fibronectin Monoclonal Antibody(M9)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: Fibronectin Monoclonal Antibody(M9)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: Fibronectin Monoclonal Antibody(M9)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: Fibronectin Monoclonal Antibody(M9)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Rat liver tissue

1: Fibronectin Monoclonal Antibody(M9) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse liver tissue

1: Fibronectin Monoclonal Antibody(M9) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


Welcome to AntibodyPlus!