Mouse Monoclonal GAPDH antibody (STJ98494)

$379.00

Reactivity: Human, Mouse, Rat, Chicken, Pig, Rabbit
Applications: WB
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal GAPDH antibody (STJ98494)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB

Recommended dilution: WB 1:1000-1:2000

Recommended protocols: check protocols

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Alternative names:

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GAPDH antibody, GAPD antibody, CDABP0047 antibody, OK/SW-cl.12 antibody,|Aging associated gene 9 protein antibody|Epididymis secretory sperm binding protein Li 162eP antibody|G3P_HUMAN antibody|G3PD antibody|G3PDH antibody|GAPD antibody|GAPDH antibody|Glyceraldehyde 3 phosphate dehydrogenase antibody|Glyceraldehyde-3-phosphate dehydrogenase antibody|HEL S 162eP antibody|Peptidyl-cysteine S-nitrosylase GAPDH antibody|Anti-GAPDH antibody [mAbcam 9484] – Loading Control (ab9484)
SCBT cat No: sc-47724|sc-59540|sc-32233|sc-51907|sc-69778|sc-81545|sc-66163|sc-20358|sc-137179|sc-166545|sc-25778|sc-365062|sc-166574|sc-48166|sc-48167|sc-31915|sc-20356|sc-20357|

 

Name

GAPDH Monoclonal Antibody

Catalogue No.

STJ98494

Reactivity

Human, Mouse, Rat, Chicken, Pig, Rabbit

Specificity

GAPDH Monoclonal Antibody detects endogenous levels of GAPDH protein.

Immunogen

Purified recombinant human GAPDH (Internal) protein fragments expressed in Ecoli

Host

Mouse

Applications

WB

Recommended dilution

WB 1:1000-1:2000

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

GAPDH Antibody was tube-contained. Purified in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.

Concentration

1 mg/ml

Purification

GAPDH Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Glyceraldehyde-3-phosphate dehydrogenase antibody, GAPDH antibody, Peptidyl-cysteine S-nitrosylase GAPDH antibody,

Database links

Human UniProt/Swiss-Prot:P04406;Mouse UniPort/Swiss-Prot: P16858;Rat UniProt/Swiss-Port: P04797;Human Entrez Gene: 2597;Mouse Entrez Gene: 14433;Rat Entrez Gene: Rn.129558

Protein names

Glyceraldehyde-3-phosphate dehydrogenase , GAPDH , Peptidyl-cysteine S-nitrosylase GAPDH ,

Protein function

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3′-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. / D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. /

Protein sequence and domain

The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. / Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Protein post-translational modifications

S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus (By similarity). S-nitrosylation of Cys-247 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity. / ISGylated. / Sulfhydration at Cys-152 increases catalytic activity. / Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at Met-46 may play a pivotal role in the formation of these insoluble structures. This modification has been detected in vitro following treatment with free radical donor (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide. It has been proposed to destabilize nearby residues, increasing the likelihood of secondary oxidative damages, including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and aggregation.

Protein cellular localization

Cytoplasm > cytosol / Nucleus / Cytoplasm > perinuclear region / Membrane / Cytoplasm > cytoskeleton

Research area

All research areas>Signaling Intermediates>GAPDH
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize GAPDH Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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