Mouse Monoclonal GATA-1 antibody [4F5] (STJ98098)

$379.00

Reactivity: Human
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal GATA-1 antibody [4F5] (STJ98098)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:10000

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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GATA1 antibody, ERYF1 antibody, GF1 antibody,|Anemia, X-linked, without thrombocytopenia, included antibody|ERYF 1 antibody|Eryf1 antibody|Erythroid transcription factor antibody|Erythrold transcription factor 1 antibody|GATA 1 antibody|GATA binding factor 1 antibody|GATA binding protein 1 (globin transcription factor 1) antibody|GATA binding protein 1 antibody|GATA-1 antibody|GATA-binding factor 1 antibody|GATA1 antibody|GATA1_HUMAN antibody|GF 1 antibody|GF-1 antibody|GF1 antibody|Globin transcription factor 1 antibody|NF E1 antibody|NF E1 DNA binding protein antibody|NF-E1 DNA-binding protein antibody|NFE 1 antibody|NFE1 antibody|Nuclear factor erythroid 1 antibody|Transcription factor GATA1 antibody|XLANP antibody|XLTDA antibody|XLTT antibody|Anti-GATA1 antibody (ab28839)
SCBT cat No: sc-1233|sc-13053|sc-1234|sc-266|sc-265|sc-267|

 

Name

GATA-1 Monoclonal Antibody

Catalogue No.

STJ98098

Reactivity

Human

Specificity

GATA-1 Monoclonal Antibody detects endogenous levels of GATA-1 protein.

Immunogen

Purified recombinant fragment of human GATA-1 expressed in E Coli

Host

Mouse

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:10000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG2b

Formulation

GATA-1 Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.

Concentration

1 mg/ml

Purification

GATA-1 Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Erythroid transcription factor antibody, Eryf1 antibody, GATA-binding factor 1 antibody, GATA-1 antibody, GF-1 antibody, NF-E1 DNA-binding protein antibody

Database links

Human UniProt/Swiss-Prot:P15976;Mouse UniPort/Swiss-Prot: P17679;Rat UniProt/Swiss-Port: P43429;Human Entrez Gene: 2623;Mouse Entrez Gene: 14460;Rat Entrez Gene: Rn.10024

Protein names

Erythroid transcription factor , Eryf1 , GATA-binding factor 1 , GATA-1 , GF-1 , NF-E1 DNA-binding protein

Protein function

Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5′-[AT]GATA[AG]-3′ within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS .

Protein tissue specificity

Erythrocytes.

Involvement in disease

X-linked dyserythropoietic anemia and thrombocytopenia (XDAT) [MIM:300367]: Disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Thrombocytopenia with beta-thalassemia, X-linked (XLTT) [MIM:314050]: An unusual form of thrombocytopenia associated with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced (hemo)globin chain synthesis resembling that of beta-thalassemia minor. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Anemia without thrombocytopenia, X-linked (XLAWT) [MIM:300835]: A form of anemia characterized by abnormal morphology of erythrocytes and granulocytes in peripheral blood, bone marrow dysplasia with hypocellularity of erythroid and granulocytic lineages, and normal or increased number of megakaryocytes. Neutropenia of a variable degree is present in affected individuals. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

The two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding (By similarity). / Contains 2 GATA-type zinc fingers.

Protein post-translational modifications

Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137 (By similarity). / Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity (By similarity). / Acetylated at 2 conserved lysine-rich motifs by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo (By similarity). Acetylated on Lys-233, Lys-245 Lys-246 by EP300.

Protein cellular localization

Nucleus

Research area

All research areas>Transcription Regulators>GATA
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize GATA-1 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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