Mouse Monoclonal Gfi-1 antibody [5D7] (STJ98107)

$379.00

Reactivity: Human
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Gfi-1 antibody [5D7] (STJ98107)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:10000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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GFI1 antibody, ZNF163 antibody,|GFI 1 antibody|GFI1 antibody|GFI1_HUMAN antibody|Growth factor independence 1 antibody|Growth factor independent 1 antibody|Growth factor independent protein 1 antibody|SCN2 antibody|Zinc finger protein 163 antibody|Zinc finger protein Gfi 1 antibody|Zinc finger protein Gfi-1 antibody|Zinc finger protein Gfi1 antibody|ZNF 163 antibody|ZNF163 antibody|Anti-GFI1 antibody (ab21061)
SCBT cat No: sc-101053|sc-376949|sc-373960|sc-22796|sc-6357|sc-8558|

 

Name

Gfi-1 Monoclonal Antibody

Catalogue No.

STJ98107

Reactivity

Human

Specificity

Gfi-1 Monoclonal Antibody detects endogenous levels of Gfi-1 protein.

Immunogen

Purified recombinant fragment of human Gfi-1 expressed in E Coli

Host

Mouse

Applications

WB, ELISA

Recommended dilution

WB 1:500-1:2000; ELISA 1:10000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

Gfi-1 Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.

Concentration

1 mg/ml

Purification

Gfi-1 Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Zinc finger protein Gfi-1 antibody, Growth factor independent protein 1 antibody, Zinc finger protein 163 antibody

Database links

Human UniProt/Swiss-Prot:Q99684;Mouse UniPort/Swiss-Prot: P70338;Rat UniProt/Swiss-Port: Q07120;Human Entrez Gene: 2672;Mouse Entrez Gene: 14581;Rat Entrez Gene: Rn.10935

Protein names

Zinc finger protein Gfi-1 , Growth factor independent protein 1 , Zinc finger protein 163

Protein function

Transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific manner. Binds to 5′-TAAATCAC[AT]GCA-3′ in the promoter region of a large number of genes. Component of several complexes, including the EHMT2-GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development. Mediates, together with U2AF1L4, the alternative splicing of CD45 and controls T-cell receptor signaling. Regulates the endotoxin-mediated Toll-like receptor (TLR) inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to regulate ITGB1-dependent neurite growth. Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of GFI1 by ZBTB17 to the CDKNIA/p21 and CDKNIB promoters. Required for the maintenance of inner ear hair cells.

Protein tissue specificity

Down-regulated by TGFB1.

Involvement in disease

Neutropenia, severe congenital 2, autosomal dominant (SCN2) [MIM:613107]: A disorder of hematopoiesis characterized by maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA) [MIM:607847]: Relatively mild form of neutropenia diagnosed in adults, but predisposing to leukemia in a subset of patients. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Zinc fingers 3, 4 and 5 are required for DNA-binding and for interaction with SPI1. / The SNAG domain of GFIs is required for nuclear location and for interaction with some corepressors. / Contains 6 C2H2-type zinc fingers.

Protein post-translational modifications

Ubiquitinated. Ubiquitination and degradation by the proteasome is inhibited by the ubiquitin ligase, ARIH2.

Protein cellular localization

Nucleus

Research area

All research areas>Transcription Regulators>Gfi
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize Gfi-1 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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