Mouse Monoclonal HAO1 antibody (STJ97395)

$99.00$319.00

Reactivity: Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal HAO1 antibody (STJ97395)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB

Recommended dilution: WB 1:1000-2000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for HAO1 Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

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HAO1 antibody, GOX1 antibody, HAOX1 antibody,|(S) 2 hydroxy acid oxidase antibody|Glycolate oxidase antibody|GOX antibody|GOX1 antibody|HAO1 antibody|HAOX1 antibody|HAOX1_HUMAN antibody|Hydroxyacid oxidase 1 antibody|MGC142225 antibody|MGC142227 antibody|OTTHUMP00000030231 antibody|Anti-HAO1 antibody (ab194790)
SCBT cat No: sc-85589|

Name

HAO1 Monoclonal Antibody

Catalogue No.

STJ97395

Reactivity

Mouse, Rat

Specificity

The antibody detects endogenous HAO1 protein.

Immunogen

Recombinant Protein

Host

Mouse

Applications

WB

Recommended dilution

WB 1:1000-2000

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

HAO1 Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

HAO1 Antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Hydroxyacid oxidase 1 antibody, HAOX1 antibody, Glycolate oxidase antibody, GOX antibody

Database links

Human UniProt/Swiss-Prot:Q9UJM8;Mouse UniPort/Swiss-Prot: Q9WU19;Rat UniProt/Swiss-Port: B0BNF9;Human Entrez Gene: 54363;Mouse Entrez Gene: 15112;Rat Entrez Gene: Rn.126636

Protein names

Hydroxyacid oxidase 1 , HAOX1 , Glycolate oxidase , GOX

Protein function

Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate. / (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2. / FMN / 141 µM for glycolate / 40 µM for 2-hydroxy octanoate / 2200 µM for glyoxylate /

Protein tissue specificity

Liver.

Protein sequence and domain

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. / Contains 1 FMN hydroxy acid dehydrogenase domain.

Protein cellular localization

Peroxisome

Research area

All research areas>Synthesis and Degradation>HAO
(View all antibody categories related to Synthesis and Degradation)

Note

AntibodyPlus can customize HAO1 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human appendix tissue

1: HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human appendix tissue

1: HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human appendix tissue

1: HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human breast cancer tissue

1: HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human breast cancer tissue

1: HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human breast cancer tissue

1: HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach cancer tissue

1: HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach cancer tissue

1: HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human stomach cancer tissue

1: HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: HAO1 Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human liver tissue

1: HAO1 Monoclonal Antibody(Mix) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human uterus cancer tissue

1: HAO1 Monoclonal Antibody(Mix) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human colon tissue

1: HAO1 Monoclonal Antibody(Mix) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat liver tissue

1: HAO1 Monoclonal Antibody(Mix) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: HAO1 Monoclonal Antibody(Mix) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse heart tissue

1: HAO1 Monoclonal Antibody(Mix) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse liver tissue

1: HAO1 Monoclonal Antibody(Mix) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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