Mouse Monoclonal HDAC1 antibody [4E1] (STJ97722)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal HDAC1 antibody [4E1] (STJ97722)

Supplier: St John’s Laboratory Ltd.

Recommended applications: IHC

Recommended dilution: IHC 1:100-200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for HDAC1 Mouse Monoclonal Antibody(4E1).

Alternative names:

Check alternative names for the antibody

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HDAC1 antibody, RPD3L1 antibody,|DKFZp686H12203 antibody|GON 10 antibody|HD1 antibody|HDAC 1 antibody|HDAC1 antibody|HDAC1_HUMAN antibody|Histone deacetylase 1 antibody|Reduced potassium dependency yeast homolog like 1 antibody|RPD3 antibody|RPD3L1 antibody|Anti-HDAC1 antibody – ChIP Grade
SCBT cat No: sc-81598|sc-6298|sc-8410|sc-7872|sc-6299|

Name

HDAC1 Mouse Monoclonal Antibody(4E1)

Catalogue No.

STJ97722

Reactivity

Human, Mouse, Rat

Specificity

HDAC1 Mouse Monoclonal Antibody(4E1) detects endogenous levels of HDAC1

Immunogen

Synthetic Peptide of HDAC1

Host

Mouse

Applications

IHC

Recommended dilution

IHC 1:100-200

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

HDAC1 Antibody(4E1) was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

HDAC1 Mouse Antibody(4E1) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Histone deacetylase 1 antibody, HD1 antibody,

Database links

Human UniProt/Swiss-Prot:Q13547;Mouse UniPort/Swiss-Prot: O09106;Rat UniProt/Swiss-Port: Q4QQW4;Human Entrez Gene: 3065;Mouse Entrez Gene: 433759;Rat Entrez Gene: Rn.1863

Protein names

Histone deacetylase 1 , HD1 ,

Protein function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates ‘Lys-310’ in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation. / Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.

Protein tissue specificity

Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.

Protein sequence and domain

Belongs to the histone deacetylase family. HD type 1 subfamily.

Protein post-translational modifications

Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1. / Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes. Phosphorylated by CDK5. / Ubiquitinated by CHFR, leading to its degradation by the proteasome. Ubiquitinated by KCTD11, leading to proteasomal degradation.

Protein cellular localization

Nucleus

Research area

All research areas>Transcription Regulators>HDAC
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize HDAC1 Antibody(4E1) according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Rat spinal cord tissue

1: HDAC1 Mouse Monoclonal Antibody(4E1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat spinal cord tissue

1: HDAC1 Mouse Monoclonal Antibody(4E1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat brain tissue

1: HDAC1 Mouse Monoclonal Antibody(4E1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat brain tissue

1: HDAC1 Mouse Monoclonal Antibody(4E1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse brain tissue

1: HDAC1 Mouse Monoclonal Antibody(4E1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse brain tissue

1: HDAC1 Mouse Monoclonal Antibody(4E1)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Rat spinal cord tissue

1: HDAC1 Mouse Monoclonal Antibody(4E1) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse brain tissue

1: HDAC1 Mouse Monoclonal Antibody(4E1) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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