Mouse Monoclonal Histone H2B antibody (STJ97064)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Histone H2B antibody (STJ97064)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB

Recommended dilution: WB 1:1000-3000

Recommended protocols: check protocols

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Click or hover above images to see image description for Histone H2B Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

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HIST1H2BB antibody, H2BFF antibody,||
SCBT cat No: To be updated

Name

Histone H2B Monoclonal Antibody

Catalogue No.

STJ97064

Reactivity

Human, Mouse, Rat

Specificity

The antibody detects endogenous Histone H2B protein.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

WB

Recommended dilution

WB 1:1000-3000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

Histone H2B Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

Histone H2B Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Histone H2B type 1-B antibody, Histone H2B.1 antibody, Histone H2B.f antibody, H2B/f antibody

Database links

Human UniProt/Swiss-Prot:P33778;Mouse UniPort/Swiss-Prot: Q64475;Rat UniProt/Swiss-Port: A0A0G2JXE0;Human Entrez Gene: 3018;Mouse Entrez Gene: 319178;

Protein names

Histone H2B type 1-B , Histone H2B.1 , Histone H2B.f , H2B/f

Protein function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Protein sequence and domain

Belongs to the histone H2B family.

Protein post-translational modifications

Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 ‘Lys-4’ (H3K4me) and ‘Lys-79’ (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 ‘Lys-4’ and ‘Lys-79’ methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. / Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription (By similarity). Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. / GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes (By similarity). / Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.

Protein cellular localization

Nucleus / Chromosome

Research area

All research areas>Transcription Regulators>Histone
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize Histone H2B Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human liver cancer tissue

1: Histone H2B Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: Histone H2B Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: Histone H2B Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse kidney tissue

1: Histone H2B Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse kidney tissue

1: Histone H2B Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse kidney tissue

1: Histone H2B Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat heart tissue

1: Histone H2B Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat heart tissue

1: Histone H2B Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat heart tissue

1: Histone H2B Monoclonal Antibody(Mix)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.


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