Mouse Monoclonal HSP70 antibody [3G10] (STJ96944)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal HSP70 antibody [3G10] (STJ96944)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IF

Recommended dilution: WB 1:1000-2000; IF 1:100-200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for HSP70 Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

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HSPA1L antibody,|Heat shock 70 kDa protein 1 Hom antibody|Heat shock 70 kDa protein 1 like antibody|Heat shock 70 kDa protein 1-Hom antibody|Heat shock 70 kDa protein 1-like antibody|Heat shock 70 kDa protein 1L antibody|Heat shock 70kD protein like 1 antibody|HS71L_HUMAN antibody|HSP70 1L antibody|HSP70 HOM antibody|HSP70-Hom antibody|HSPA1L antibody|hum70t antibody|Spermatid specific heat shock protein 70 antibody|Anti-HSPA1L antibody (ab154409)
SCBT cat No: sc-367639|

Name

HSP70 Monoclonal Antibody

Catalogue No.

STJ96944

Reactivity

Human, Mouse, Rat

Specificity

The antibody detects endogenous HSP70 proteins.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

WB, IF

Recommended dilution

WB 1:1000-2000; IF 1:100-200

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

HSP70 Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

HSP70 Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Heat shock 70 kDa protein 1-like antibody, Heat shock 70 kDa protein 1L antibody, Heat shock 70 kDa protein 1-Hom antibody, HSP70-Hom antibody

Database links

Human UniProt/Swiss-Prot:P34931 ;Human Entrez Gene: 3305;

Protein names

Heat shock 70 kDa protein 1-like , Heat shock 70 kDa protein 1L , Heat shock 70 kDa protein 1-Hom , HSP70-Hom

Protein function

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage (By similarity). Positive regulator of PARK2 translocation to damaged mitochondria.

Protein tissue specificity

Expressed in spermatids.

Protein sequence and domain

Belongs to the heat shock protein 70 family.

Research area

All research areas>Signaling Intermediates>Apg Heat Shock Protein
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize HSP70 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human breast cancer tissue

1: HSP70 Monoclonal Antibody(3G10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human breast cancer tissue

1: HSP70 Monoclonal Antibody(3G10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human breast cancer tissue

1: HSP70 Monoclonal Antibody(3G10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse lung tissue

1: HSP70 Monoclonal Antibody(3G10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse lung tissue

1: HSP70 Monoclonal Antibody(3G10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse lung tissue

1: HSP70 Monoclonal Antibody(3G10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat testis tissue

1: HSP70 Monoclonal Antibody(3G10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat testis tissue

1: HSP70 Monoclonal Antibody(3G10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat testis tissue

1: HSP70 Monoclonal Antibody(3G10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human uterus cancer tissue

1: HSP70 Monoclonal Antibody(3G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human Tonsil tissue

1: HSP70 Monoclonal Antibody(3G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human colon tissue

1: HSP70 Monoclonal Antibody(3G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat testis tissue

1: HSP70 Monoclonal Antibody(3G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat lung tissue

1: HSP70 Monoclonal Antibody(3G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: HSP70 Monoclonal Antibody(3G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat spinal cord tissue

1: HSP70 Monoclonal Antibody(3G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat spleen tissue

1: HSP70 Monoclonal Antibody(3G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse lung tissue

1: HSP70 Monoclonal Antibody(3G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue

1: HSP70 Monoclonal Antibody(3G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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