Mouse Monoclonal HSP90beta antibody (STJ97032)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal HSP90beta antibody (STJ97032)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB

Recommended dilution: WB 1:1000-3000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for HSP90beta Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

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HSP90AB1 antibody, HSP90B antibody, HSPC2 antibody, HSPCB antibody,|Heat shock 86 kDa antibody|Heat shock protein 90kDa alpha cytosolic class A member 1 antibody|Heat shock protein 90kDa alpha cytosolic class B member 1 antibody|Heat shock protein HSP 90 alpha antibody|Heat shock protein HSP 90 beta antibody|Heat shock protein HSP 90-alpha antibody|HS90A_HUMAN antibody|HSP 84 antibody|HSP 86 antibody|Hsp 90 antibody|HSP86 antibody|HSP90A antibody|HSP90AA1 antibody|HSP90AB1 antibody|HSP90B antibody|HSPC1 antibody|HSPC2 antibody|HSPCAL1 antibody|HSPCAL4 antibody|Renal carcinoma antigen NY-REN-38 antibody|Anti-Hsp90 antibody [AC88] SCBT cat No: sc-515081|sc-382636|sc-59577|sc-13119|sc-7947|sc-1055|sc-59578|

Name

HSP90beta Monoclonal Antibody

Catalogue No.

STJ97032

Reactivity

Human, Mouse, Rat

Specificity

The antibody detects endogenous HSP90beta protein.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

WB

Recommended dilution

WB 1:1000-3000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

HSP90beta Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

HSP90beta Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Heat shock protein HSP 90-beta antibody, HSP 90 antibody, Heat shock 84 kDa antibody, HSP 84 antibody, HSP84 antibody

Database links

Human UniProt/Swiss-Prot:P08238;Mouse UniPort/Swiss-Prot: P11499;Rat UniProt/Swiss-Port: P34058;Human Entrez Gene: 3326;Mouse Entrez Gene: 15516;Rat Entrez Gene: Rn.98667

Protein names

Heat shock protein HSP 90-beta , HSP 90 , Heat shock 84 kDa , HSP 84 , HSP84

Protein function

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.

Protein sequence and domain

The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins. / Belongs to the heat shock protein 90 family.

Protein post-translational modifications

Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro). / ISGylated. / S-nitrosylated; negatively regulates the ATPase activity.

Protein cellular localization

Cytoplasm / Melanosome

Research area

All research areas>Signaling Intermediates>HSP
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize HSP90beta Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Mouse spleen tissue

1: HSP90beta Monoclonal Antibody(M2)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: HSP90beta Monoclonal Antibody(M2)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: HSP90beta Monoclonal Antibody(M2)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat lung tissue

1: HSP90beta Monoclonal Antibody(M2)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat lung tissue

1: HSP90beta Monoclonal Antibody(M2)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat lung tissue

1: HSP90beta Monoclonal Antibody(M2)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat spleen tissue

1: HSP90beta Monoclonal Antibody(M2)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat spleen tissue

1: HSP90beta Monoclonal Antibody(M2)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat spleen tissue

1: HSP90beta Monoclonal Antibody(M2)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human Tonsil tissue

1: HSP90beta Monoclonal Antibody(M2) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human colon tissue

1: HSP90beta Monoclonal Antibody(M2) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: HSP90beta Monoclonal Antibody(M2) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat heart tissue

1: HSP90beta Monoclonal Antibody(M2) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat testis tissue

1: HSP90beta Monoclonal Antibody(M2) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: HSP90beta Monoclonal Antibody(M2) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat brain tissue

1: HSP90beta Monoclonal Antibody(M2) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat spleen tissue

1: HSP90beta Monoclonal Antibody(M2) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse testis tissue

1: HSP90beta Monoclonal Antibody(M2) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue

1: HSP90beta Monoclonal Antibody(M2) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse brain tissue

1: HSP90beta Monoclonal Antibody(M2) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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