Mouse Monoclonal IkappaB beta antibody [1F3] (STJ97539)

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Reactivity: Human, Rat, Mouse
Applications: IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal IkappaB beta antibody [1F3] (STJ97539)

Supplier: St John’s Laboratory Ltd.

Recommended applications: IHC

Recommended dilution: IHC 1:100-200

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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NFKBIB antibody, IKBB antibody, TRIP9 antibody,|I kappa B beta antibody|I-kappa-B-beta antibody|IkappaBbeta antibody|IKB beta antibody|IkB-B antibody|IkB-beta antibody|IKBB antibody|IKBB_HUMAN antibody|IkBbeta antibody|NF kappa BIB antibody|NF-kappa-B inhibitor beta antibody|NF-kappa-BIB antibody|Nfkbib antibody|Thyroid receptor interacting protein 9 antibody|Thyroid receptor-interacting protein 9 antibody|TR interacting protein 9 antibody|TR-interacting protein 9 antibody|TRIP-9 antibody|TRIP9 antibody|Anti-IKB beta antibody (ab7547)
SCBT cat No: sc-74453|

Name

IkappaB beta Monoclonal Antibody

Catalogue No.

STJ97539

Reactivity

Human, Rat, Mouse

Specificity

IkappaB beta Mouse Monoclonal Antibody(1F3) detects endogenous levels of IkappaB beta

Immunogen

Recombinant Protein

Host

Mouse

Applications

IHC

Recommended dilution

IHC 1:100-200

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

IkappaB beta Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

IkappaB beta Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

NF-kappa-B inhibitor beta antibody, NF-kappa-BIB antibody, I-kappa-B-beta antibody, IkB-B antibody, IkB-beta antibody, IkappaBbeta antibody, Thyroid receptor-interacting protein 9 antibody, TR-interacting protein 9 antibody, TRIP-9 antibody

Database links

Human UniProt/Swiss-Prot:Q15653;Mouse UniPort/Swiss-Prot: Q60778;Rat UniProt/Swiss-Port: Q5U342;Human Entrez Gene: 4793;Mouse Entrez Gene: 18036;Rat Entrez Gene: Rn.8395

Protein names

NF-kappa-B inhibitor beta , NF-kappa-BIB , I-kappa-B-beta , IkB-B , IkB-beta , IkappaBbeta , Thyroid receptor-interacting protein 9 , TR-interacting protein 9 , TRIP-9

Protein function

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.

Protein tissue specificity

Expressed in all tissues examined.

Protein sequence and domain

Belongs to the NF-kappa-B inhibitor family. / Contains 6 ANK repeats.

Protein post-translational modifications

Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.

Protein cellular localization

Cytoplasm / Nucleus

Research area

All research areas>Transcription Regulators>I κ B
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize IkappaB beta Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human liver cancer tissue

1: IkappaB beta Monoclonal Antibody(1F3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human liver cancer tissue

1: IkappaB beta Monoclonal Antibody(1F3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse testis tissue

1: IkappaB beta Monoclonal Antibody(1F3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse testis tissue

1: IkappaB beta Monoclonal Antibody(1F3)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: IkappaB beta Monoclonal Antibody(1F3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach tissue

1: IkappaB beta Monoclonal Antibody(1F3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: IkappaB beta Monoclonal Antibody(1F3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat spleen tissue

1: IkappaB beta Monoclonal Antibody(1F3) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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