Mouse Monoclonal Ikaros antibody (STJ98508)

$379.00

Reactivity: Human, Mouse, Dog
Applications: WB
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Ikaros antibody (STJ98508)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB

Recommended dilution: WB 1:1000-1:2000

Recommended protocols: check protocols

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Alternative names:

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IKZF1 antibody, IK1 antibody, IKAROS antibody, LYF1 antibody, ZNFN1A1 antibody,|CLL associated antigen KW 6 antibody|DNA-binding protein Ikaros antibody|hIk 1 antibody|Hs.54452 antibody|IK1 antibody|Ikaros (zinc finger protein) antibody|IKAROS antibody|IKAROS family zinc finger 1 (Ikaros) antibody|Ikaros family zinc finger protein 1 antibody|Ikzf1 antibody|IKZF1_HUMAN antibody|LYF1 antibody|Lymphoid transcription factor LyF-1 antibody|PRO0758 antibody|Zinc finger protein subfamily 1A 1 (Ikaros) antibody|Zinc finger protein subfamily 1A 1 antibody|Zinc finger protein, subfamily 1A, member 1 antibody|ZNFN1A1 antibody|Anti-Ikaros antibody (ab26083)
SCBT cat No: sc-398265|sc-9861|sc-13039|sc-9859|

 

Name

Ikaros Monoclonal Antibody

Catalogue No.

STJ98508

Reactivity

Human, Mouse, Dog

Specificity

Ikaros Monoclonal Antibody detects endogenous levels of Ikaros protein.

Immunogen

Purified recombinant human Ikaros (C-terminus) protein fragments expressed in Ecoli

Host

Mouse

Applications

WB

Recommended dilution

WB 1:1000-1:2000

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

Ikaros Antibody was tube-contained. Purified in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.

Concentration

1 mg/ml

Purification

Ikaros Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

DNA-binding protein Ikaros antibody, Ikaros family zinc finger protein 1 antibody, Lymphoid transcription factor LyF-1 antibody

Database links

Human UniProt/Swiss-Prot:Q13422;Rat UniProt/Swiss-Port: D4A9W4;Human Entrez Gene: 10320;Mouse Entrez Gene: 22778;Rat Entrez Gene: Rn.218270

Protein names

DNA-binding protein Ikaros , Ikaros family zinc finger protein 1 , Lymphoid transcription factor LyF-1

Protein function

Transcription regulator of hematopoietic cell differentiation . Binds gamma-satellite DNA . Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms .

Protein tissue specificity

Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.

Involvement in disease

Note: Defects in IKZF1 are frequent occurrences (28.6%) in acute lymphoblasic leukemia (ALL). Such alterations or deletions lead to poor prognosis for ALL.; Note: Chromosomal aberrations involving IKZF1 are a cause of B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation t(3;7)(q27;p12), with BCL6.; Immunodeficiency, common variable, 13 (CVID13) [MIM:616873]: A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. CVID13 is an autosomal dominant disease associated with a striking decrease in B-cell numbers. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin. / The C-terminal zinc-finger domain is required for dimerization. / Belongs to the Ikaros C2H2-type zinc-finger protein family. / Contains 6 C2H2-type zinc fingers.

Protein post-translational modifications

Phosphorylation controls cell-cycle progression from late G1 stage to S stage. Hyperphosphorylated during G2/M phase. Dephosphorylated state during late G1 phase. Phosphorylation on Thr-140 is required for DNA and pericentromeric location during mitosis. CK2 is the main kinase, in vitro. GSK3 and CDK may also contribute to phosphorylation of the C-terminal serine and threonine residues. Phosphorylation on these C-terminal residues reduces the DNA-binding ability. Phosphorylation/dephosphorylation events on Ser-13 and Ser-295 regulate TDT expression during thymocyte differentiation. Dephosphorylation by protein phosphatase 1 regulates stability and pericentromeric heterochromatin location. Phosphorylated in both lymphoid and non-lymphoid tissues (By similarity). Phosphorylation at Ser-361 and Ser-364 downstream of SYK induces nuclear translocation. / Sumoylated. Simulataneous sumoylation on the 2 sites results in a loss of both HDAC-dependent and HDAC-independent repression. Has no effect on pericentromeric heterochromatin location. Desumoylated by SENP1 (By similarity). / Polyubiquitinated.

Protein cellular localization

Nucleus / Nucleus / Cytoplasm

Research area

All research areas>Transcription Regulators>Ikaros
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize Ikaros Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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