Mouse Monoclonal IKKalpha antibody [3G12H9] (STJ98163)

$379.00

Reactivity: Human
Applications: WB, IF, FC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal IKKalpha antibody [3G12H9] (STJ98163)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IF, FC, ELISA

Recommended dilution: WB 1:500-1:2000; IF 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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CHUK antibody, IKKA antibody, TCF16 antibody,|chuk antibody|CHUK1 antibody|Conserved Helix Loop Helix Ubiquitous Kinase antibody|Conserved helix loop ubiquitous kinase antibody|Conserved helix-loop-helix ubiquitous kinase antibody|I Kappa B Kinase 1 antibody|I Kappa B Kinase Alpha antibody|I-kappa-B kinase 1 antibody|I-kappa-B kinase alpha antibody|IkappaB kinase antibody|IkB kinase alpha subunit antibody|IkBKA antibody|IKK 1 antibody|IKK A antibody|IKK a kinase antibody|IKK-A antibody|IKK-alpha antibody|IKK1 antibody|IKKA antibody|IKKA_HUMAN antibody|Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody|Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibody|Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody|NFKBIKA antibody|Nuclear Factor Kappa B Inhibitor Kinase Alpha antibody|Nuclear factor NF kappa B inhibitor kinase alpha antibody|Nuclear factor NF-kappa-B inhibitor kinase alpha antibody|Nuclear factor NFkappaB inhibitor kinase alpha antibody|Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody|TCF-16 antibody|TCF16 antibody|Transcription factor 16 antibody|Anti-IKK alpha antibody [Y463] (ab32041)
SCBT cat No: sc-7190|

 

Name

IKKalpha Monoclonal Antibody

Catalogue No.

STJ98163

Reactivity

Human

Specificity

IKKalpha Monoclonal Antibody detects endogenous levels of IKKalpha protein.

Immunogen

Purified recombinant fragment of human IKKalpha expressed in E Coli

Host

Mouse

Applications

WB, IF, FC, ELISA

Recommended dilution

WB 1:500-1:2000; IF 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

IKKalpha Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.

Concentration

1 mg/ml

Purification

IKKalpha Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody, I-kappa-B kinase alpha antibody, IKK-A antibody, IKK-alpha antibody, IkBKA antibody, IkappaB kinase antibody, Conserved helix-loop-helix ubiquitous kinase antibody, I-kappa-B kinase 1 antibody, IKK1 antibody, Nuclear factor NF-kappa-B inhibitor kinase alpha antibody, NFKBIKA antibody, Transcription factor 16 antibody, TCF-16 antibody

Database links

Human UniProt/Swiss-Prot:O15111;Rat UniProt/Swiss-Port: G3V926;Human Entrez Gene: 1147;Mouse Entrez Gene: 12675;Rat Entrez Gene: Rn.23019

Protein names

Inhibitor of nuclear factor kappa-B kinase subunit alpha , I-kappa-B kinase alpha , IKK-A , IKK-alpha , IkBKA , IkappaB kinase , Conserved helix-loop-helix ubiquitous kinase , I-kappa-B kinase 1 , IKK1 , Nuclear factor NF-kappa-B inhibitor kinase alpha , NFKBIKA , Transcription factor 16 , TCF-16

Protein function

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at ‘Ser-10’ that are subsequently acetylated at ‘Lys-14’ by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. Also phosphorylates FOXO3 and may regulate this pro-apoptotic transcription factor . / ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein]. / Activated when phosphorylated and inactivated when dephosphorylated.

Protein tissue specificity

Widely expressed.

Involvement in disease

Cocoon syndrome (COCOS) [MIM:613630]: A lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

The kinase domain is located in the N-terminal region. The leucine zipper is important to allow homo- and hetero-dimerization. At the C-terminal region is located the region responsible for the interaction with NEMO/IKBKG. / Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily. / Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated. / (Microbial infection) Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.

Protein cellular localization

Cytoplasm / Nucleus

Research area

All research areas>Kinases and Phosphatases>IKK alpha
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize IKKalpha Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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