Mouse Monoclonal JNK3 antibody [10E4A4] (STJ98192)

$379.00

Reactivity: Human, Mouse
Applications: WB, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal JNK3 antibody [10E4A4] (STJ98192)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:10000

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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MAPK10 antibody, JNK3 antibody, JNK3A antibody, PRKM10 antibody, SAPK1B antibody,|JNK 46 antibody|JNK 55 antibody|MAPK10 antibody|MAPK8 antibody|MAPK9 antibody|Mitogen-activated protein kinase 10 antibody|Mitogen-activated protein kinase 8 antibody|Mitogen-activated protein kinase 9 antibody|PRKM10 antibody|PRKM8 antibody|PRKM9 antibody|SAPK antibody|SAPK1 antibody|SAPK1b antibody|Phospho anti-JNK1 + JNK2 + JNK3 (T183+T183+T221) antibody [EPR5693] (ab124956)
SCBT cat No: sc-130075|

 

Name

JNK3 Monoclonal Antibody

Catalogue No.

STJ98192

Reactivity

Human, Mouse

Specificity

JNK3 Monoclonal Antibody detects endogenous levels of JNK3 protein.

Immunogen

Purified recombinant fragment of human JNK3 (aa28-233) expressed in E Coli

Host

Mouse

Applications

WB, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:10000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

JNK3 Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.

Concentration

1 mg/ml

Purification

JNK3 Antibody was purified using affinity purification.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Mitogen-activated protein kinase 10 antibody, MAP kinase 10 antibody, MAPK 10 antibody, MAP kinase p49 3F12 antibody, Stress-activated protein kinase 1b antibody, SAPK1b antibody, Stress-activated protein kinase JNK3 antibody, c-Jun N-terminal kinase 3 antibody

Database links

Human UniProt/Swiss-Prot:P53779;Mouse UniPort/Swiss-Prot: Q61831;Rat UniProt/Swiss-Port: A0A0U1RVI2;Human Entrez Gene: 5602;Mouse Entrez Gene: 26414;Rat Entrez Gene: Rn.9911

Protein names

Mitogen-activated protein kinase 10 , MAP kinase 10 , MAPK 10 , MAP kinase p49 3F12 , Stress-activated protein kinase 1b , SAPK1b , Stress-activated protein kinase JNK3 , c-Jun N-terminal kinase 3

Protein function

Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the beta-amyloid precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock . / ATP + a protein = ADP + a phosphoprotein. / Mg2+ / Activated by threonine and tyrosine phosphorylation by two dual specificity kinases, MAP2K4 and MAP2K7. MAP2K7 phosphorylates MAPK10 on Thr-221 causing a conformational change and a large increase in Vmax. MAP2K4 then phosphorylates Tyr-223 resulting in a further increase in Vmax. Inhibited by dual specificity phosphatases, such as DUSP1. Inhibited by HDAC9.

Protein tissue specificity

Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.

Involvement in disease

Note: A chromosomal aberration involving MAPK10 has been found in a single patient with pharmacoresistant epileptic encephalopathy. Translocation t(Y;4)(q11.2;q21) which causes MAPK10 truncation. .

Protein sequence and domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. / Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. / Contains 1 protein kinase domain.

Protein post-translational modifications

Dually phosphorylated on Thr-221 and Tyr-223 by MAP2K4 and MAP2K7, which activates the enzyme. MAP2K7 shows a strong preference for Thr-221 while MAP2K4 phosphorylates Tyr-223 preferentially. Weakly autophosphorylated on threonine and tyrosine residues in vitro. / Palmitoylation regulates subcellular location and axonal development.

Protein cellular localization

Cytoplasm / Membrane / Lipid-anchor / Nucleus / Mitochondrion

Research area

All research areas>Kinases and Phosphatases>JNK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize JNK3 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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