Mouse Monoclonal Ki 67 antibody [4A8] (STJ96966)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Ki 67 antibody [4A8] (STJ96966)

Supplier: St John’s Laboratory Ltd.

Recommended applications: IHC

Recommended dilution: IHC 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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MKI67 antibody,|Antigen identified by monoclonal antibody Ki 67 antibody|Protein phosphatase 1 regulatory subunit 105 antibody|RP11-380J17.2 antibody|Anti-Ki67 antibody (ab15580)
SCBT cat No: sc-7844|sc-15402|sc-23900|sc-7846|sc-56319|sc-101861|

Name

Ki 67 Monoclonal Antibody

Catalogue No.

STJ96966

Reactivity

Human, Mouse, Rat

Specificity

The antibody detects endogenous Ki 67 proteins.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

IHC

Recommended dilution

IHC 1:200

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

Ki 67 Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

Ki 67 Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Proliferation marker protein Ki-67 antibody, Antigen identified by monoclonal antibody Ki-67 antibody, Antigen KI-67 antibody, Antigen Ki67 antibody

Database links

Human UniProt/Swiss-Prot:P46013;Rat UniProt/Swiss-Port: D4A0Y6;Human Entrez Gene: 4288;Mouse Entrez Gene: 17345;Rat Entrez Gene: Rn.233802

Protein names

Proliferation marker protein Ki-67 , Antigen identified by monoclonal Ki-67 , Antigen KI-67 , Antigen Ki67

Protein function

Thought to be required for maintaining cell proliferation.

Protein tissue specificity

Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.

Protein sequence and domain

Contains 1 FHA domain.

Protein cellular localization

Nucleus / Nucleus > nucleolus / Chromosome

Note

AntibodyPlus can customize Ki 67 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human breast cancer tissue

1: Ki 67 Monoclonal Antibody(4A8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human breast cancer tissue

1: Ki 67 Monoclonal Antibody(4A8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse testis tissue

1: Ki 67 Monoclonal Antibody(4A8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse testis tissue

1: Ki 67 Monoclonal Antibody(4A8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat brain tissue

1: Ki 67 Monoclonal Antibody(4A8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat brain tissue

1: Ki 67 Monoclonal Antibody(4A8)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human uterus cancer tissue

1: Ki 67 Monoclonal Antibody(4A8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: Ki 67 Monoclonal Antibody(4A8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: Ki 67 Monoclonal Antibody(4A8) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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