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Mouse Monoclonal Ku-80 antibody [5C5] (STJ98211)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, IF, FC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
XRCC5 antibody, G22P2 antibody,|86 kDa subunit of Ku antigen antibody|KUB 2 antibody|KUB2 antibody|Lupus Ku autoantigen protein p86 antibody|NFIV antibody|Nuclear factor IV antibody|Thyroid lupus autoantigen antibody|Thyroid-lupus autoantigen antibody|TLAA antibody|X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody|X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody|X-ray repair cross-complementing protein 5 antibody|Xray repair complementing defective repair in Chinese hamster cells 5 antibody|XRCC 5 antibody|XRCC5 antibody|XRCC5_HUMAN antibody|Anti-Ku80 antibody [5C5] (ab119935)
SCBT cat No: sc-53021|sc-135964|sc-5280|sc-1484|sc-9034|sc-33653|sc-1485|sc-56135|sc-56136|
Ku-80 Monoclonal Antibody
|Catalogue No.|| |
Ku-80 Monoclonal Antibody detects endogenous levels of Ku-80 protein.
Purified recombinant fragment of human Ku-80 expressed in E Coli
WB, IHC, IF, FC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; FC 1:200-1:400; ELISA 1:10000
Ku-80 Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.
Ku-80 Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
X-ray repair cross-complementing protein 5 antibody, 86 kDa subunit of Ku antigen antibody, ATP-dependent DNA helicase 2 subunit 2 antibody, ATP-dependent DNA helicase II 80 kDa subunit antibody, CTC box-binding factor 85 kDa subunit antibody, CTC85 antibody, CTCBF antibody, DNA repair protein XRCC5 antibody, Ku80 antibody, Ku86 antibody, Lupus Ku autoantigen protein p86 antibody, Nuclear factor IV antibody, Thyroid-lupus autoantigen antibody, TLAA antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 antibody, double-strand-break rejoining antibody
|Protein names|| |
X-ray repair cross-complementing protein 5 , 86 kDa subunit of Ku antigen , ATP-dependent DNA helicase 2 subunit 2 , ATP-dependent DNA helicase II 80 kDa subunit , CTC box-binding factor 85 kDa subunit , CTC85 , CTCBF , DNA repair protein XRCC5 , Ku80 , Ku86 , Lupus Ku autoantigen protein p86 , Nuclear factor IV , Thyroid-lupus autoantigen , TLAA , X-ray repair complementing defective repair in Chinese hamster cells 5 , double-strand-break rejoining
|Protein function|| |
Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3′-5′ direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together . The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression . The XRCC5/6 dimer probably also acts as a 5′-deoxyribose-5-phosphate lyase (5′-dRP lyase), by catalyzing the beta-elimination of the 5′ deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5′-dRP activity, and allows to ‘clean’ the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription .
|Protein tissue specificity|| |
Expression increases during promyelocyte differentiation.
|Protein sequence and domain|| |
The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage. / Belongs to the ku80 family. / Contains 1 Ku domain.
|Protein post-translational modifications|| |
Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity. / Sumoylated. / Ubiquitinated by RNF8 via ‘Lys-48’-linked ubiquitination following DNA damage, leading to its degradation and removal from DNA damage sites . Ubiquitinated by RNF138, leading to remove the Ku complex from DNA breaks .
|Protein cellular localization|| |
Nucleus / Nucleus > nucleolus / Chromosome
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St John’s Laboratory Ltd.
|Product type|| |
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