Mouse Monoclonal Lactoferrin antibody (STJ97062)

$99.00$319.00

Reactivity: Human
Applications: IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal Lactoferrin antibody (STJ97062)

Supplier: St John’s Laboratory Ltd.

Recommended applications: ELISA

Recommended dilution: ELISA: 1:5000-10000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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LTF antibody, GIG12 antibody, LF antibody,|GIG12 antibody|Growth inhibiting protein 12 antibody|HLF2 antibody|Kaliocin 1 antibody|Lactoferrin antibody|Lactoferroxin-C antibody|Lactotransferrin antibody|LF antibody|LTF antibody|Neutrophil lactoferrin antibody|Talalactoferrin antibody|TRFL_HUMAN antibody|Anti-Lactoferrin antibody [2B8] (ab10110)
SCBT cat No: sc-52048|sc-52694|sc-53498|sc-14434|sc-25622|sc-101487|sc-80549|sc-14431|

Name

Lactoferrin Monoclonal Antibody

Catalogue No.

STJ97062

Reactivity

Human

Specificity

The antibody detects endogenous Lactoferrin protein.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

ELISA

Recommended dilution

ELISA: 1:5000-10000

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

Lactoferrin Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

Lactoferrin Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Lactotransferrin antibody, Lactoferrin antibody, Growth-inhibiting protein 12 antibody, Talalactoferrin antibody

Database links

Human UniProt/Swiss-Prot:P02788;Mouse UniPort/Swiss-Prot: P08071;Rat UniProt/Swiss-Port: D3ZAB1;Human Entrez Gene: 4057;Mouse Entrez Gene: 17002;Rat Entrez Gene: Rn.218647

Protein names

Lactotransferrin , Lactoferrin , Growth-inhibiting protein 12 , Talalactoferrin

Protein function

Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. / Lactotransferrin is a major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions. Has antimicrobial activity, which depends on the extracellular cation concentration. Antimicrobial properties include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane. Can also prevent bacterial biofilm development in P.aeruginosa infection. Has weak antifungal activity against C.albicans. Has anabolic, differentiating and anti-apoptotic effects on osteoblasts and can also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth. Promotes binding of species C adenoviruses to epithelial cells, promoting adenovirus infection. Can inhibit papillomavirus infections. Stimulates the TLR4 signaling pathway leading to NF-kappa-B activation and subsequent pro-inflammatory cytokine production while also interfering with the lipopolysaccharide (LPS)-stimulated TLR4 signaling. Inhibits neutrophil granulocyte migration to sites of apoptosis, when secreted by apoptotic cells. Stimulates VEGFA-mediated endothelial cell migration and proliferation. Binds heparin, chondroitin sulfate and possibly other glycosaminoglycans (GAGs). Also binds specifically to pneumococcal surface protein A (pspA), the lipid A portion of bacterial lipopolysaccharide (LPS), lysozyme and DNA. / Lactoferricin binds to the bacterial surface and is crucial for the bactericidal functions. Has some antiviral activity against papillomavirus infection. N-terminal region shows strong antifungal activity against C.albicans. Contains two BBXB heparin-binding consensus sequences that appear to form the predominate functional GAG-binding site. / Kaliocin-1 has antimicrobial activity and is able to permeabilize different ions through liposomal membranes. / Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. / The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. / Isoform DeltaLf: transcription factor with antiproliferative properties and ability to induce cell cycle arrest. Binds to the DeltaLf response element found in the SKP1, BAX, DCPS, and SELH promoters. / Preferential at -Arg-Ser-Arg-Arg-, – and -Arg-Arg-Ser-Arg-, -, and of Z-Phe-Arg-, -aminomethylcoumarin.

Protein tissue specificity

High levels are found in saliva and tears, intermediate levels in serum and plasma, and low levels in urine. In kidney, detected in the distal collecting tubules in the medulla but not in the cortical region or in blood vessels. Detected in peripheral blood neutrophils (at protein level). Isoform 1 and isoform DeltaLf are expressed in breast, prostate, spleen, pancreas, kidney, small intestine, lung, skeletal muscle, uterus, thymus and fetal liver. Isoform 1 is expressed in brain, testis and peripheral blood leukocytes; isoform DeltaLf is barely detectable in these tissues. Isoform DeltaLf is expressed in placenta, liver and ovary; isoform 1 is barely detectable in these tissues. In kidney, isoform 1 is expressed at high levels in the collecting tubules of the medulla but at very low levels in the cortex.

Protein sequence and domain

Belongs to the transferrin family. / Contains 2 transferrin-like domains.

Protein post-translational modifications

Isoform DeltaLf: Ubiquitinated at Lys-379 and Lys-391. / Poly-N-acetyllactosaminic carbohydrate moiety seems to be needed for TLR4 activation.

Protein cellular localization

Secreted / Cytoplasmic granule / Cytoplasm / Nucleus

Research area

All research areas>Transport and Trafficking>lactoferrin
(View all antibody categories related to Transport and Trafficking)

Note

AntibodyPlus can customize Lactoferrin Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human lung cancer tissue

1: Lactoferrin Monoclonal Antibody(Q100)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung cancer tissue

1: Lactoferrin Monoclonal Antibody(Q100)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human breast cancer tissue

1: Lactoferrin Monoclonal Antibody(Q100) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue

1: Lactoferrin Monoclonal Antibody(Q100) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human kidney tissue

1: Lactoferrin Monoclonal Antibody(Q100) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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