Mouse Monoclonal LC3A antibody [5G10] (STJ97755)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal LC3A antibody [5G10] (STJ97755)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC

Recommended dilution: WB 1:1000-2000; IHC 1:100-200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for LC3A Mouse Monoclonal Antibody(5G10).

Alternative names:

Check alternative names for the antibody

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MAP1LC3A antibody,|ATG8E antibody|LC3 antibody|LC3A antibody|MAP1 light chain 3 like protein 1 antibody|MAP1 light chain 3-like protein 1 antibody|MAP1A/1B light chain 3 A antibody|MAP1A/MAP1B LC3 A antibody|MAP1A/MAP1B light chain 3 A antibody|MAP1ALC3 antibody|MAP1BLC3 antibody|Map1lc3a antibody|Microtubule associated proteins 1A/1B light chain 3 antibody|Microtubule-associated protein 1 light chain 3 alpha antibody|Microtubule-associated proteins 1A and 1B, light chain 3 antibody|Microtubule-associated proteins 1A/1B light chain 3A antibody|MLP3A_HUMAN antibody|Anti-MAP1LC3A antibody [EP1983Y] – Autophagosome Marker (ab52768)
SCBT cat No: sc-134226|sc-16756|

Name

LC3A Mouse Monoclonal Antibody(5G10)

Catalogue No.

STJ97755

Reactivity

Human, Mouse, Rat

Specificity

LC3A Mouse Monoclonal Antibody(5G10) detects endogenous levels of LC3A

Immunogen

Synthetic Peptide of LC3A

Host

Mouse

Applications

WB, IHC

Recommended dilution

WB 1:1000-2000; IHC 1:100-200

Clonality

Monoclonal

Conjugation

Unconjugated

Formulation

LC3A Antibody(5G10) was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

LC3A Mouse Antibody(5G10) was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Microtubule-associated proteins 1A/1B light chain 3A antibody, Autophagy-related protein LC3 A antibody, Autophagy-related ubiquitin-like modifier LC3 A antibody, MAP1 light chain 3-like protein 1 antibody, MAP1A/MAP1B light chain 3 A antibody, MAP1A/MAP1B LC3 A antibody, Microtubule-associated protein 1 light chain 3 alpha antibody

Database links

Human UniProt/Swiss-Prot:Q9H492;Mouse UniPort/Swiss-Prot: Q91VR7;Rat UniProt/Swiss-Port: Q6XVN8;Human Entrez Gene: 84557;Mouse Entrez Gene: 66734;Rat Entrez Gene: Rn.3135

Protein names

Microtubule-associated proteins 1A/1B light chain 3A , Autophagy-related protein LC3 A , Autophagy-related ubiquitin-like modifier LC3 A , MAP1 light chain 3-like protein 1 , MAP1A/MAP1B light chain 3 A , MAP1A/MAP1B LC3 A , Microtubule-associated protein 1 light chain 3 alpha

Protein function

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) . Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation .

Protein tissue specificity

Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.

Protein sequence and domain

Belongs to the ATG8 family.

Protein post-translational modifications

The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II . / The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. / Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.

Protein cellular localization

Cytoplasm > cytoskeleton / Endomembrane system; Lipid-anchor / Cytoplasmic vesicle > autophagosome membrane; Lipid-anchor / Cytoplasmic vesicle > autophagosome

Note

AntibodyPlus can customize LC3A Antibody(5G10) according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


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Antibody customer reviews & validation data - 27

Validation data ID: 27

Submission date: 2016-11-29

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Result figure:
Result figure

Figure description:

Rat thyrocytes were treated with TSH receptor antibody for 24 hrs., fixed, permeabilized and then stained with primary antibodies. After washing FAR red conjugated secondary antibody was used and then analyzed by LI-COR imaging.

Product name

Beclin-1, MAP LC3beta, LC3A, IL-6, IL-1beta

Product catalog No.

STJ97761, STJ97398, STJ97755, STJ96713, STJ96566

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Western Blot

Antibody specificity rating

Excellent

Antibody overall rating

Good

Your comments

Mouse Monoclonal Beclin-1 antibody [5C2] (STJ97761)
Rabbit Polyclonal MAP LC3beta antibody (STJ97398)
Mouse Monoclonal LC3A antibody [5G10] (STJ97755)
Rabbit Polyclonal IL-6 antibody (STJ96713)
Rabbit Polyclonal IL-1beta antibody (STJ96566)
In fact, I have used them in LI-COR IN CELL WESTERN method. They worked nicely. The method was according to the instructions provided with the LI-COR kit with their buffers and antibodies.

Detailed protocol

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Antibody customer reviews & validation data - 18

Validation data ID: 18

Submission date: 2016-11-23

View Details

Result figure:
Result figure

Figure description:

specifically stained photoreceptor inner segment.

Result figure:
Result figure

Figure description:

Clean and specific signal at expected MW

Product name

Mouse monoclonal LC3A antibody (5G10)

Product catalog No.

STJ97755

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Immunofluorescence (IF)

Antibody specificity rating

Excellent

Antibody overall rating

Excellent

Your comments

good LC3A antibody, clean signal both in IF and Western

Detailed protocol

View Details

view database submit reviews
 

Immunofluorescence analysis of Human lung cancer tissue

1: LC3A Mouse Monoclonal Antibody(5G10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Human lung cancer tissue

1: LC3A Mouse Monoclonal Antibody(5G10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: LC3A Mouse Monoclonal Antibody(5G10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: LC3A Mouse Monoclonal Antibody(5G10)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human uterus tissue

1: LC3A Mouse Monoclonal Antibody(5G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human uterus cancer tissue

1: LC3A Mouse Monoclonal Antibody(5G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human Tonsil tissue

1: LC3A Mouse Monoclonal Antibody(5G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: LC3A Mouse Monoclonal Antibody(5G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue

1: LC3A Mouse Monoclonal Antibody(5G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach tissue

1: LC3A Mouse Monoclonal Antibody(5G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue

1: LC3A Mouse Monoclonal Antibody(5G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: LC3A Mouse Monoclonal Antibody(5G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat spleen tissue

1: LC3A Mouse Monoclonal Antibody(5G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse testis tissue

1: LC3A Mouse Monoclonal Antibody(5G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse colon tissue

1: LC3A Mouse Monoclonal Antibody(5G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue

1: LC3A Mouse Monoclonal Antibody(5G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse spleen tissue

1: LC3A Mouse Monoclonal Antibody(5G10) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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