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Mouse Monoclonal Lck antibody [8E5F9; 4B2C7] (STJ98215)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:10000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
LCK antibody,|IMD22 antibody|LCK antibody|Lck p56 antibody|LCK proto-oncogene, Src family tyrosine kinase antibody|LCK_HUMAN antibody|Leukocyte C-terminal Src kinase antibody|LSK antibody|Lymphocyte cell specific protein tyrosine kinase antibody|Lymphocyte cell-specific protein-tyrosine kinase antibody|Lymphocyte specific protein tyrosine kinase antibody|Membrane associated protein tyrosine kinase antibody|Oncogene lck antibody|P56 LCK antibody|p56(LSTRA) protein tyrosine kinase antibody|p56-LCK antibody|p56lck antibody|pp58 lck antibody|pp58lck antibody|Protein YT16 antibody|Proto oncogene tyrosine protein kinase LCK antibody|Proto-oncogene Lck antibody|Protooncogene tyrosine protein kinase LCK antibody|T cell specific protein tyrosine kinase antibody|T cell-specific protein-tyrosine kinase antibody|T lymphocyte specific protein tyrosine kinase p56lck antibody|Tyrosine-protein kinase Lck antibody|YT 16 antibody|YT16 antibody|Anti-Lck antibody [Y123] (ab32149)
SCBT cat No: sc-71498|sc-13|sc-135971|sc-433|sc-166627|sc-166628|sc-28882|sc-32014|sc-65579|sc-135972|
Lck Monoclonal Antibody
|Catalogue No.|| |
Lck Monoclonal Antibody detects endogenous levels of Lck protein.
Purified recombinant fragment of human Lck expressed in E Coli
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:10000
Lck Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.
Lck Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Tyrosine-protein kinase Lck antibody, Leukocyte C-terminal Src kinase antibody, LSK antibody, Lymphocyte cell-specific protein-tyrosine kinase antibody, Protein YT16 antibody, Proto-oncogene Lck antibody, T cell-specific protein-tyrosine kinase antibody, p56-LCK antibody
|Protein names|| |
Tyrosine-protein kinase Lck , Leukocyte C-terminal Src kinase , LSK , Lymphocyte cell-specific protein-tyrosine kinase , Protein YT16 , Proto-oncogene Lck , T cell-specific protein-tyrosine kinase , p56-LCK
|Protein function|| |
Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / The relative activities of the inhibitory tyrosine-protein kinase CSK and the activating tyrosine-protein phosphatase PTPRC/CD45 determine the level of LCK activity. These interactions allow rapid and efficient activation of LCK in response to TCR stimulation.
|Protein tissue specificity|| |
Expressed specifically in lymphoid cells.
|Involvement in disease|| |
Note: A chromosomal aberration involving LCK is found in leukemias. Translocation t(1;7)(p34;q34) with TCRB.; Immunodeficiency 22 (IMD22) [MIM:615758]: A primary immunodeficiency characterized by T-cell dysfunction. Affected individuals present with lymphopenia, recurrent infections, severe diarrhea, and failure to thrive. . Note: The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation. / Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. / Contains 1 protein kinase domain. / Contains 1 SH2 domain. / Contains 1 SH3 domain.
|Protein post-translational modifications|| |
Autophosphorylated on Tyr-394, increasing enzymatic activity, this site is dephosphorylated by PTN22. Phosphorylated on Tyr-505 by CSK, decreasing activity. Dephosphorylated by PTPRC/CD45. Dephosphorylation at Tyr-394 by PTPN2 negatively regulates T-cell receptor signaling. / Myristoylation is required prior to palmitoylation. / Palmitoylation regulates subcellular location.
|Protein cellular localization|| |
Cytoplasm / Cell membrane / Lipid-anchor / Cytoplasmic side
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St John’s Laboratory Ltd.
|Product type|| |
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