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Mouse Monoclonal LKB1 antibody [4H12] (STJ98220)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, FC, ELISA
Recommended dilution: WB 1:500-1:2000; FC 1:200-1:400; ELISA 1:10000
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
STK11 antibody, LKB1 antibody, PJS antibody,|hLKB1 antibody|Renal carcinoma antigen NY-REN-19 antibody|Serine/Threonine Kinase 11 antibody|Serine/threonine protein kinase 11 antibody|Serine/threonine protein kinase LKB1 antibody|Serine/threonine protein kinase STK11 antibody|Serine/threonine-protein kinase 11 antibody|Serine/threonine-protein kinase LKB1 antibody|Serine/threonine-protein kinase XEEK1 antibody|Stk11 antibody|STK11_HUMAN antibody|Anti-LKB1 antibody [Ley 37D/G6] (ab15095)
SCBT cat No: sc-5637|sc-5638|
LKB1 Monoclonal Antibody
|Catalogue No.|| |
Human, Mouse, Monkey
LKB1 Monoclonal Antibody detects endogenous levels of LKB1 protein.
Purified recombinant fragment of human LKB1 expressed in E Coli
WB, FC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; FC 1:200-1:400; ELISA 1:10000
LKB1 Antibody was tube-contained. Ascitic fluid containing 0.03% sodium azide.
LKB1 Antibody was purified using affinity purification.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Serine/threonine-protein kinase STK11 antibody, Liver kinase B1 antibody, LKB1 antibody, hLKB1 antibody, Renal carcinoma antigen NY-REN-19 antibody
|Protein names|| |
Serine/threonine-protein kinase STK11 , Liver kinase B1 , LKB1 , hLKB1 , Renal carcinoma antigen NY-REN-19
|Protein function|| |
Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. In vein endothelial cells, inhibits PI3K/Akt signaling activity and thus induces apoptosis in response to the oxidant peroxynitrite (in vitro). Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with NUAK1, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair . / Isoform 2: Has a role in spermiogenesis. / ATP + a protein = ADP + a phosphoprotein. / Mg2+, Mn2+ / Activated by forming a complex with STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): STRADA (or STRADB)-binding promotes a conformational change of STK11/LKB1 in an active conformation, which is stabilized by CAB39/MO25alpha (or CAB39L/MO25beta) interacting with the STK11/LKB1 activation loop. Sequestration in the nucleus by NR4A1 prevents it from phosphorylating and activating cytoplasmic AMPK.
|Protein tissue specificity|| |
Ubiquitously expressed. Strongest expression in testis and fetal liver.
|Involvement in disease|| |
Peutz-Jeghers syndrome (PJS) [MIM:175200]: An autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Testicular germ cell tumor (TGCT) [MIM:273300]: A common malignancy in males representing 95% of all testicular neoplasms. TGCTs have various pathologic subtypes including: unclassified intratubular germ cell neoplasia, seminoma (including cases with syncytiotrophoblastic cells), spermatocytic seminoma, embryonal carcinoma, yolk sac tumor, choriocarcinoma, and teratoma. . Note: The gene represented in this entry may be involved in disease pathogenesis.; Note: Defects in STK11 are associated with some sporadic cancers, especially lung cancers. Frequently mutated and inactivated in non-small cell lung cancer (NSCLC). Defects promote lung cancerigenesis process, especially lung cancer progression and metastasis. Confers lung adenocarcinoma the ability to trans-differentiate into squamous cell carcinoma. Also able to promotes lung cancer metastasis, via both cancer-cell autonomous and non-cancer-cell autonomous mechanisms.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Phosphorylated by ATM at Thr-363 following ionizing radiation (IR). Phosphorylation at Ser-428 by RPS6KA1 and/or some PKA is required to inhibit cell growth. Phosphorylation at Ser-428 is also required during neuronal polarization to mediate phosphorylation of BRSK1 and BRSK2 (By similarity). Phosphorylation by PKC/PRKCZ at Ser-428 promotes peroxynitrite-induced nuclear export of STK11, leading to PTEN activation and subsequent inhibition of AKT signaling. Phosphorylation by PKC/PRKCZ at Ser-399 in isoform 2 promotes metformin (or peroxynitrite)-induced nuclear export of STK11 and activation of AMPK. UV radiation-induced phosphorylation at Thr-363 mediates CDKN1A degradation (By similarity). / Acetylated. Deacetylation at Lys-48 enhances cytoplasmic localization and kinase activity in vitro.
|Protein cellular localization|| |
Nucleus / Cytoplasm / Membrane / Mitochondrion / Nucleus / Cytoplasm
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St John’s Laboratory Ltd.
|Product type|| |
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