Mouse Monoclonal MAP2 antibody [7D4] (STJ96969)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Mouse Monoclonal MAP2 antibody [7D4] (STJ96969)

Supplier: St John’s Laboratory Ltd.

Recommended applications: IHC

Recommended dilution: IHC 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for MAP2 Monoclonal Antibody.

Alternative names:

Check alternative names for the antibody

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MAP2 antibody,|DKFZp686I2148 antibody|MAP 2 antibody|MAP dendrite specific antibody|MAP-2 antibody|MAP2 antibody|MAP2A antibody|MAP2B antibody|MAP2C antibody|Microtubule associated protein 2 antibody|Microtubule-associated protein 2 antibody|MTAP2_HUMAN antibody|Anti-MAP2 antibody (ab5392)
SCBT cat No: sc-135979|sc-74421|sc-74422|sc-80012|sc-80013|sc-32791|sc-74420|sc-390543|sc-5359|sc-74419|sc-20172|sc-5357|sc-56561|

Name

MAP2 Monoclonal Antibody

Catalogue No.

STJ96969

Reactivity

Human, Mouse, Rat

Specificity

The antibody detects endogenous MAP2 proteins.

Immunogen

Synthetic Peptide

Host

Mouse

Applications

IHC

Recommended dilution

IHC 1:200

Clonality

Monoclonal

Conjugation

Unconjugated

Isotype

IgG1

Formulation

MAP2 Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Purification

MAP2 Antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Microtubule-associated protein 2 antibody, MAP-2 antibody

Database links

Human UniProt/Swiss-Prot:P11137;Mouse UniPort/Swiss-Prot: P20357;Rat UniProt/Swiss-Port: Q64715;Human Entrez Gene: 4133;Mouse Entrez Gene: 17756;Rat Entrez Gene: Rn.10484

Protein names

Microtubule-associated protein 2 , MAP-2

Protein function

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

Protein sequence and domain

Contains 3 Tau/MAP repeats.

Protein post-translational modifications

Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2/MAP2c is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.

Protein cellular localization

Cytoplasm > cytoskeleton

Note

AntibodyPlus can customize MAP2 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Mouse brain tissue

1: MAP2 Monoclonal Antibody(7D4)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse brain tissue

1: MAP2 Monoclonal Antibody(7D4)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: MAP2 Monoclonal Antibody(7D4)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: MAP2 Monoclonal Antibody(7D4)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Mouse spleen tissue

1: MAP2 Monoclonal Antibody(7D4)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat brain tissue

1: MAP2 Monoclonal Antibody(7D4)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat brain tissue

1: MAP2 Monoclonal Antibody(7D4)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat brain tissue

1: MAP2 Monoclonal Antibody(7D4)(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human Tonsil tissue

1: MAP2 Monoclonal Antibody(7D4) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat kidney tissue

1: MAP2 Monoclonal Antibody(7D4) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat brain tissue

1: MAP2 Monoclonal Antibody(7D4) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse brain tissue

1: MAP2 Monoclonal Antibody(7D4) was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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